Our work owes its origin to a recent note of Ram Murty [‘Irrationality of zeros of the digamma function’, Number Theory in Memory of Eduard Wirsing (eds. H. Maier, R. Steuding and J. Steuding) (Springer, Cham, 2023), 237–243], in which he proves that all the zeros of the digamma function are irrational with at most one possible exception. We extend this investigation to higher-order polygamma functions.