TOXOPLASMA GONDII AND TOXOPLASMOSIS

Toxoplasma gondii is an obligate intracellular protozoan parasite that belongs to the phylum Apicomplexa (Dubey et al. Reference Dubey, Miller and Frenkel1970). This phylum includes important protozoan parasites such as Plasmodium spp., Cryptosporidium spp. and Eimeria spp. among others. Toxoplasma gondii is the causative agent of toxoplasmosis, a disease spread worldwide with about 500 million to a billion people chronically infected (Tenter et al. Reference Tenter, Heckeroth and Weiss2000). Toxoplasma gondii is able to infect almost any nucleated cell of warm-blooded animals, including mammals and birds (Dubey et al. Reference Dubey, Miller and Frenkel1970).

Toxoplasma gondii may undergo both cycles of sexual reproduction within the definitive host (any member of the cat family) and cycles of asexual reproduction within any warm-blooded animal, including humans (Dubey et al. Reference Dubey, Miller and Frenkel1970). Inside intermediate hosts, T. gondii presents two stages of asexual development: the rapidly growing tachyzoites, responsible for the acute illness, and the slowly dividing bradyzoites, responsible for the chronic and asymptomatic infection (Dubey et al. Reference Dubey, Lindsay and Speer1998). Tachyzoites invade many cell types and rapidly multiply by repeated endodyogeny inside a parasitophorous vacuole (PV) until disruption of the host cell and the subsequent invasion of nearby cells (Dubey et al. Reference Dubey, Lindsay and Speer1998). In response to stress factors, usually in reaction to the host immune system, tachyzoites can convert to bradyzoites, forming tissue cysts which may persist as latent cysts for the rest of the host's life (Dubey et al. Reference Dubey, Lindsay and Speer1998). Within tissue cysts, bradyzoites replicate slowly by endodyogeny (Dubey et al. Reference Dubey, Miller and Frenkel1970). Cysts can be ingested by a definitive host, in which case the parasite undergoes cycles of sexual reproduction (Dubey et al. Reference Dubey, Miller and Frenkel1970).

Toxoplasmosis is of medical importance because T. gondii can cause opportunistic diseases in immunocompromised individuals, due to reactivation of a latent infection. Toxoplasma gondii can also cause spontaneous abortion or congenital birth defects in newborns if the mother is primo-infected during pregnancy (Carlier et al. Reference Carlier, Truyens, Deloron and Peyron2012). The active form of the parasite can cause encephalitis and neurological diseases, and can also affect heart, liver, inner ears and eyes (chorioretinitis). Recently, toxoplasmosis has been linked with schizophrenia and other neurological disorders and behaviour alterations (Miman et al. Reference Miman, Mutlu, Ozcan, Atambay, Karlidag and Unal2010; Park et al. Reference Park, Kwon, Jeong, Lee, Hwangbo, Yoon and Shim2012; Halonen and Weiss, Reference Halonen and Weiss2013). Despite the existence of effective drug regimens, in some cases, the therapy is not well tolerated by the patients. In addition, there is still no effective therapy against latent infection. It is expected that specific proteins of the parasite, which play important or essential roles in T. gondii pathogenesis, allow the generation of novel therapies that can overcome some of the afore-mentioned situations. In this regard, the chaperone heat shock protein 90 (Hsp90) has arisen as an interesting drug target against acute and latent toxoplasmosis (Shonhai et al. Reference Shonhai, Maier, Przyborski and Blatch2011; Angel et al. Reference Angel, Matrajt and Echeverria2013; Roy et al. Reference Roy, Nageshan, Ranade and Tatu2012; Rochani et al. Reference Rochani, Singh and Tatu2013).

Hsp90: STRUCTURE AND COMPLEXES

Hsp90 is a widely distributed and highly conserved molecular chaperone that is ubiquitously expressed throughout nature, being one of the most abundant proteins within non-stressed cells (Picard, Reference Picard2002; Pratt and Toft, Reference Pratt and Toft2003; Tsutsumi et al. Reference Tsutsumi, Mollapour, Graf, Lee, Scroggins, Xu, Haslerova, Hessling, Konstantinova, Trepel, Panaretou, Buchner, Mayer, Prodromou and Neckers2009). Rather than binding any unfolded protein, Hsp90 binds more than 200 client proteins which require it for the correct activation of key cellular processes such as control of genetic expression, cell cycle progression, apoptosis, cancer, stress response, plant immunity, development and even evolutionary processes (McClellan et al. Reference McClellan, Xia, Deutschbauer, Davis, Gerstein and Frydman2007; Bogumil and Dagan, Reference Bogumil and Dagan2012; Jackson, Reference Jackson2013). Hsp90 is an ATP-dependent chaperone whose ATPase activity is regulated by the binding of co-chaperones and substrates (Prodromou and Pearl, Reference Prodromou and Pearl2003). This binding can be specifically inhibited by ansamycin-benzoquinone antibiotics (Geldanamycin–GA- and its derivatives) and Radicicol. These antibiotics bind specifically to the Hsp90 ATP binding pocket with higher affinity than ATP itself (Stebbins et al. Reference Stebbins, Russo, Schneider, Rosen, Hartl and Pavletich1997). GA specifically inhibits the ATPase activity of Hsp90 as a result of its competition with ATP for binding to the N-terminal domain nucleotide binding pocket (Prodromou et al. Reference Prodromou, Roe, O'Brien, Ladbury, Piper and Pearl1997). This mechanism interferes with the maturation process of client proteins, facilitating their ubiquitin-mediated proteasomal degradation (Pearl et al. Reference Pearl, Prodromou and Workman2008). This can be used as an important tool for the analysis of Hsp90 functions and the identification of its client proteins.

The Hsp90 structure consists of three highly conserved domains: an N-terminal domain (ATP binding site, GA binding site, p23, Aha 1 and Cdc37 co-chaperones binding site), a middle domain (catalytic loop required for ATPase activity, Aha1 co-chaperone binding site) and a C-terminal domain (dimerization interface) (Retzlaff et al. Reference Retzlaff, Hagn, Mitschke, Hessling, Gugel, Kessler, Richter and Buchner2010; Jackson, Reference Jackson2013). In addition, it has a charged linker region and the MEEVD motif (which binds TPR-containing co-chaperones). Several co-chaperones (e.g. p50/Cdc37, p60Hop/Sti1, p23, Aha1) modulate Hsp90 ATPase activity, thereby altering the cycling rate of the chaperone machine (Li et al. Reference Li, Soroka and Buchner2012). The composition of co-chaperone complexes varies according to the client proteins and the pathway in which the chaperone is involved (Li and Buchner, Reference Li and Buchner2013). In higher eukaryotes, it is possible to define at least four Hsp90 cycles: (i) the Hsp70/Hsp90 cycle or Hsp90-heterocomplex for steroid hormone receptors; (ii) the Hsp70/Hsp90 cycle or Hsp90-heterocomplex for protein kinases (which involves cdc37 protein); (iii) a cycle that involves SGT1 co-chaperones, which in plants plays a role in immune sensors, but it is also associated with the kinetochore assembly pathway; and (iv) Hsp90 complexes associated with RNA processing (Li and Buchner, Reference Li and Buchner2013). Hsp90-heterocomplexes consist of sequential complexes formed by Hsp90 and different co-chaperones. These are assembled during protein client maturation forming dynamic multi-chaperone complexes. They could be defined as early, intermediate and late Hsp70/Hsp90 cycles (Li and Buchner, Reference Li and Buchner2013).

TOXOPLASMA GONDII Hsp90 AND ITS CYCLES

Toxoplasma gondii presents two of the above-mentioned Hsp90 cycles (Fig. 1). In the case of the T. gondii Hsp90 (TgHsp90)-heterocomplex, T. gondii seems to have only one of these pathways because the cdc37 protein has not been detected. In our laboratory, we have identified all the chaperones and co-chaperones associated with TgHsp90-heterocomplex (Echeverria et al. Reference Echeverria, Figueras, Vogler, Kriehuber, de Miguel, Deng, Dalmasso, Matthews, Matrajt, Haslbeck, Buchner and Angel2010; Figueras et al. Reference Figueras, Martin, Echeverria, de Miguel, Naguleswaran, Sullivan, Corvi and Angel2012, unpublished).We have also detected an early/intermediate and a late TgHsp90 complex similar to those found in higher eukaryotes (Fig. 1) (Echeverria et al. Reference Echeverria, Figueras, Vogler, Kriehuber, de Miguel, Deng, Dalmasso, Matthews, Matrajt, Haslbeck, Buchner and Angel2010). The early/intermediate TgHsp90 complex is composed at least by HIP-Hsp70-TgHsp90 whereas the late complex is composed by p23-TgHsp90. In addition, p23 and TgHsp90 was shown to be cytosolic during the tachyzoite stage and both cytosolic and nuclear during the bradyzoite stage, whereas HIP, Sis1 and HOP was shown to be cytosolic during both stages of asexual reproduction in vitro (Echeverria et al. Reference Echeverria, Matrajt, Harb, Zappia, Costas, Roos, Dubremetz and Angel2005; Ueno et al. Reference Ueno, Dautu, Haga, Munyaka, Carmen, Kobayashi and Igarashi2011; Figueras et al. Reference Figueras, Martin, Echeverria, de Miguel, Naguleswaran, Sullivan, Corvi and Angel2012, unpublished). These data indicate that both p23 and TgHsp90 could be engaged to a multitude of large protein complexes as has been observed in higher eukaryotes (Picard et al. Reference Picard, Suslova and Briand2006).

Fig. 1. Hsp90 cycles present in T. gondii. (A) Hsp90/Hsp70 cycle or TgHsp90-heterocomplex for transcription factors/protein kinases. The compositions of the early/intermediate complex and of the late complex are shown on the left and right hand, respectively. The co-chaperone cdc37 binds to the Hsp90/Hsp70 cycle associated to kinases in higher eukaryotes. Since it was not identified in the T. gondii database, it is indicated with a dashed line. (B) The T. gondii CBF3-assembly complex. (C) The sequence domain organization of the two putative SGT1s identified in the T. gondii database. 90: TgHsp90; 70: Hsp70; HIP: Hsp70 interacting protein; HOP: Hsp70/Hsp90 organizing protein; 40: Hsp40; CP: Client Protein; Imm/PP5: Immunophilin or PP5; P23: p23; Sgt1: suppressor of G2 allele of SKP1, S-phase kinase-associated protein 1; Ctf13: Chromosome transmission fidelity protein 13; Ndc10: is also named CBF2 for centromere binding factor 2; Cep3: is also named CBF3b for centromere binding factor 3b.

The Hsp90 protein also participates in the complex involved in the early kinetochore assembly pathway, as observed in yeast (Fig. 1) (Makhnevych and Houry, Reference Makhnevych and Houry2012). Mining the T. gondii database (www.toxodb.org) it was possible to reconstruct the kinetochore assembly pathway and two putative SGT1 co-chaperones could be detected (Fig. 1). SGT1 proteins belong to a group of proteins that have different domains, including the CS (CHORD [cysteine and histidine-rich domain]-Sgt1 domain (sometimes called p23-like or alpha crystallin domain) and the SGS domain (Sgt1-specific domain) at the carboxy terminus. In addition, SGT1 proteins have a TPR domain that links Skp1 to the core of the Hsp90 chaperone (Catlett and Kaplan, Reference Catlett and Kaplan2006). Toxoplasma gondii has two putative sequences encoding SGT1, identified as TGME49_305820 and TGME49_231590 (Fig. 1C). None of them has a TPR domain but TGME49_305820 has a zinc finger domain at its amino terminus, not observed in other SGT1 proteins.

In addition to these Hsp90 cycles, this chaperone has been found to be associated with RNA processing in yeast, involving Pih1, Tah1 and Rvb1 and Rvb2 (Li and Buchner, Reference Li and Buchner2013). Mining the T. gondii database has not retrieved any orthologue of Pih1 and Tah1 (Suvorova et al. Reference Suvorova, Radke, Ting, Vinayak, Alvarez, Kratzer, Kim, Striepen and White2013). However, the data obtained from a combined analysis of co-immunoprecipitation and mass spectrometry assays using anti-TgHsp90 antibody and orthologous partners of TgHsp90 from a Plasmodium falciparum Protein-Protein Interaction (PPI) network database (see Table S1) allowed the retrieval of several putative TgHsp90 interactors involved in RNA processing. These data suggest that TgHsp90 would have an important role in this process and thus could become a very interesting field for future studies.

POST-TRANSLATIONAL MODIFICATIONS

Hsp90 function can be altered by different post-translational modifications (PTMs) such as phosphorylation, acetylation, S-nitrosylation, oxidation and ubiquitination (Mollapour and Neckers, Reference Mollapour and Neckers2012). Recently, the phosphoproteome and acetylome of T. gondii have been determined (Treeck et al. Reference Treeck, Sanders, Elias and Boothroyd2011; Jeffers and Sullivan, Reference Jeffers and Sullivan2012; Xue et al. Reference Xue, Jeffers, Sullivan and Uversky2013). These data have been incorporated in the www.toxodb.org database allowing us to generate a PTM map of TgHsp90 (Fig. 2, Table 1). Nonetheless these modifications should be further confirmed by other methods.

Fig. 2. Schematic representation of TgHsp90. In black: The N-terminal domain, in grey: the central domain and in light grey: the C-terminal domain. Highlighted are the amino acids MEEVD and the residues that are identified to have received post-translational modifications (PTMs). One letter code was used to indicate the corresponding amino acids. The panel also shows the biological processes in which TgHsp90, more likely through the TgHsp90-heterocomplex, is involved: modulation of invasion, cell cycle and differentiation.

Table 1. PTMs of T. gondii, yeast and human Hsp90 members

^a Data only include acetylated lysines identified in T. gondii and human Hsp90. Other lysines that were detected in human Hsp90 are not included.

ND: indicates not determined; Normal font: PTMs identified; italic font: orthologue residue; –: non phosphorylated (Y, T, S), acetylated (K) or S-nitrosylated (C) residue; Obs.: Hsp90 domains are only identified for phosphorylation section.

Toxoplasma gondii Hsp90 would be phosphorylated at several sites along its sequence (Table 1); most of them (except S464) are also modified in human or yeast Hsp90 orthologues (Mollapour et al. Reference Mollapour, Tsutsumi, Donnelly, Beebe, Tokita, Lee, Lee, Morra, Bourboulia, Scroggins, Colombo, Blagg, Panaretou, Stetler-Stevenson, Trepel, Piper, Prodromou, Pearl and Neckers2010a ; Mollapour and Neckers, Reference Mollapour and Neckers2012; Soroka et al. Reference Soroka, Wandinger, Mausbacher, Schreiber, Richter, Daub and Buchner2012). Interestingly, human and yeast Hsp90s have other phosphorylated sites of which PTMs was not detected in TgHsp90 despite the conserved residues in the parasite chaperone (Table 1). Although the role of Hsp90 phosphorylation in T. gondii has not yet been studied, it is known that phosphorylation has important implications in the regulation of the Hsp90 structure and function in other organisms. Mutation of S485 in yeast Hsp90 is lethal, altering the dynamics of the conformational changes (Soroka et al. Reference Soroka, Wandinger, Mausbacher, Schreiber, Richter, Daub and Buchner2012). In some cases, phosphorylation of Hsp90 reduces the avidity for its client protein. The lack of phosphorylation of S226/S255 and S231 on the linker region of HuHsp90β and HuHsp90α, respectively, increases the binding affinity of the chaperones for the transcription factor arylhydrocarbon receptor (Ogiso et al. Reference Ogiso, Kagi, Matsumoto, Nishimoto, Arai, Shirouzu, Mimura, Fujii-Kuriyama and Yokoyama2004). The treatment of v-src-transfected cells with okadaic acid, a serine/threonine phosphatase inhibitor, destabilizes the Hsp90-pp60^v−src chaperone complex (Mimnaugh et al. Reference Mimnaugh, Worland, Whitesell and Neckers1995). Mutation of T22 on yeast Hsp90 (T23 in TgHsp90, Table 1) results in the accumulation of less glucocorticoid receptor (GR) protein than wild-type cells and reduces GR and pp60^v−src activities in a yeast model (Nathan and Lindquist, Reference Nathan and Lindquist1995). More recently, it has been observed that phosphorylation of T22 in yeast Hsp90 affects its ATPase activity and also its function on several client proteins, either kinases or non-kinases, affecting the interaction between the Hsp90 protein and co-chaperones (Mollapour et al. Reference Mollapour, Tsutsumi, Kim, Trepel and Neckers2011a , Reference Mollapour, Tsutsumi, Truman, Xu, Vaughan, Beebe, Konstantinova, Vourganti, Panaretou, Piper, Trepel, Prodromou, Pearl and Neckers b ). By contrast, indirect analysis based on the inability of PP5 phosphatase to bind the Hsp90-heme-regulated inhibitor of protein synthesis (HRI) has shown that chaperone phosphorylation enhances HRI activity (Shao et al. Reference Shao, Hartson and Matts2002). Phosphorylation of tyrosine 197 in human Hsp90α (Y186 in TgHsp90, Table 1) dissociates cdc37 from Hsp90 (Xu et al. Reference Xu, Mollapour, Prodromou, Wang, Scroggins, Palchick, Beebe, Siderius, Lee, Couvillon, Trepel, Miyata, Matts and Neckers2012). This study also showed that mutation of Y627 (Y604 in T. gondii, Table 1) favours the release of client Cdk4 and co-chaperones (AHA1 and PP5). Wang et al. (Reference Wang, Song, Zhuo, Fu, Shi, Liang, Tong, Chang and Luo2009) have shown that phosphorylation of T90 by protein kinase A (PKA) stimulates the cellular secretion of human Hsp90α.

Phosphorylation of Hsp90 is mediated by several kinases such as DNA-dependent protein kinase (DNA-PK), casein kinase II (CK2), PKA, c-Src and Wee1 (Mollapour et al. Reference Mollapour, Tsutsumi and Neckers2010b ; Mollapour and Neckers Reference Mollapour and Neckers2012). The phosphorylation status is also regulated by PP5 and Ppt1 phosphatases (Mollapour et al. Reference Mollapour, Tsutsumi and Neckers2010b ). Their T. gondii putative counterparts are: CK2 (TGME49_272400); PKA (TGME49_242070); DNA-PK (TGME49_266000); NEK [Wee1] (TGME49_319700); c-Src (TGME49_272475); Ppt1 (TGME49_312200); and PP5 (TGME49_312200). None of these kinases and phosphatases was detected as TgHsp90/p23 putative interactors (Table S1). Since PP5 is an Hsp90 co-chaperone, it is expected that TgPP5 will be pulled down with TgHsp90 in future studies.

Thus, as observed, phosphorylation of Hsp90 is of key importance for Hsp90 function and complex cycle. Despite the numerous putative phosphorylated sites found in TgHsp90, there are several phosphorylated sites in yeast and human Hsp90s with important regulatory functions, which are present in TgHsp90 but not in the phosphoproteome. It is expected that new sites of phosphorylation will be detected in TgHsp90 and that their importance and biological role would be determined.

More recently, the role of a lysine acetylation in regulating Hsp90 function has been evidenced. Human Hsp90s present several acetylable lysines along their sequence (Mollapour and Neckers, Reference Mollapour and Neckers2012). The histone acetyl-transferase (HAT) and histone deacetylase (HDAC) enzymes are involved in modulating the acetylation status of Hsp90. The acetylation of Hsp90 negatively affects the Hsp90 functions. Exposure of cancer cells to the HDAC inhibitor FK228 has been shown to block the interaction of Hsp90 with the key cell cycle regulators p53 and Raf-1 (Yu et al. Reference Yu, Guo, Marcu, Neckers, Nguyen, Chen and Schrump2002). In the TgHsp90 sequence, two putative acetylated lysines (K384 and K559) have been identified (Jeffers and Sullivan, Reference Jeffers and Sullivan2012; Xue et al. Reference Xue, Jeffers, Sullivan and Uversky2013) (Fig. 2 and Table 1). These lysines are conserved in human Hsp90 and one is also acetylated (Table 1). The role of lysine K294 in human Hsp90α, also present in TgHsp90 (K271, Table 1), has been further studied. The replacement of this lysine by alanine (K294A) dramatically reduces the interaction of Hsp90 with the client receptor tyrosine kinase ErbB2 and the kinase p60^v−Src (Scroggins et al. Reference Scroggins, Robzyk, Wang, Marcu, Tsutsumi, Beebe, Cotter, Felts, Toft, Karnitz, Rosen and Neckers2007). A similar effect has been observed with co-chaperones AHA1, FKBP52, HOP, Hsp70 and C-terminus of Hsp70-interacting protein (CHIP), where the mutant has shown a reduced or undetectable level of interaction with the Hsp90 (K294A) mutant version (Scroggins et al. Reference Scroggins, Robzyk, Wang, Marcu, Tsutsumi, Beebe, Cotter, Felts, Toft, Karnitz, Rosen and Neckers2007).

The enzyme responsible for the regulation Hsp90 deacetylation is HDAC6 (class II deacetylase) whose inactivation results in Hsp90 hyper-acetylation, leading to the dissociation of the functional GR-Hsp90 and thus affecting the receptor maturation (Kovacs et al. Reference Kovacs, Murphy, Gaillard, Zhao, Wu, Nicchitta, Yoshida, Toft, Pratt and Yao2005; Murphy et al. Reference Murphy, Morishima, Kovacs, Yao and Pratt2005). HDAC1, another class II deacetylase, has also been reported to deacetylate Hsp90 in the nucleus of human breast cancer cells (Zhou et al. Reference Zhou, Agoston, Atadja, Nelson and Davidson2008), and both HDAC1 and HDAC10 inhibit the productive Hsp90 chaperoning of VEGF receptor proteins (Park et al. Reference Park, Kim, Choi, Lee, Oh, Im, Bang and Kim2008). Although HDAC6 has not been detected, other members of the HDAC class II family (such as HDAC1 and HDAC5) have been detected by mining the T. gondii database (Vanagas et al. Reference Vanagas, Jeffers, Bogado, Dalmasso, Sullivan and Angel2012). However, none of them have been identified as Hsp90 interactors so far.

Acetylation of Hsp90 seems to be headed by p300/CBP (CREB-binding protein) HATs (Kovacs et al. Reference Kovacs, Murphy, Gaillard, Zhao, Wu, Nicchitta, Yoshida, Toft, Pratt and Yao2005; Mollapour and Neckers, Reference Mollapour and Neckers2012). However, up to now, little is known about this issue. Although it is a recent field of study, the role of these PTMs in biological processes is gaining the interest of researchers, as phosphorylation did in the past. The exploration of acetylation/deacetylation modulation in TgHsp90 will bring on interesting findings in the future and will probably promote novel anti-Hsp90 drugs.

S-nitrosylation is a covalent attachment of a nitrogen monoxide group to the thiol side chain of a cysteine. This reversible PTM of Hsp90 is mediated by nitric oxide (NO), and has been shown to inhibit its chaperone activity (Martinez-Ruiz et al. Reference Martinez-Ruiz, Villanueva, Gonzalez de Orduna, Lopez-Ferrer, Higueras, Tarin, Rodriguez-Crespo, Vazquez and Lamas2005). This PTM occurs in the C-terminal domain of Hsp90α at C597, a residue also present in TgHsp90 (Table 1). The S-nitrosylation has not been studied in TgHsp90 yet, being another intriguing subject for future studies.

THE CELL CYCLE

The incubation of intracellular tachyzoites with GA has a clear effect on T. gondii reducing the replication rate of tachyzoites (Ahn et al. Reference Ahn, Kim and Nam2003; Echeverria et al. Reference Echeverria, Matrajt, Harb, Zappia, Costas, Roos, Dubremetz and Angel2005). Toxoplasma gondii replicates with a mechanism named endodyogeny, in which two daughter cells bud inside a mother cell (Gubbels et al. Reference Gubbels, White and Szatanek2008). The cell cycle of highly replicative tachyzoites comprises the Gap phase G1-S-Mitosis, whereas the G2 is not detectable (Radke et al. Reference Radke, Striepen, Guerini, Jerome, Roos and White2001). Inside the host cell, the rate of replication is about one complete cycle every 5–9 h and then starts again to complete several rounds (Radke et al. Reference Radke, Striepen, Guerini, Jerome, Roos and White2001). Although the cell cycle resembles that of higher eukaryotes, the complete round of parasite replication has some differences: the nuclear envelope does not disappear along the parasite division (Striepen et al. Reference Striepen, Jordan, Reiff and van Dooren2007) and chromosomes do not present a high order level of condensation. In addition, many of the checkpoint factors that control G1 to S phase progression in higher eukaryotes are not detected in T. gondii (Gubbels et al. Reference Gubbels, White and Szatanek2008; Behnke et al. Reference Behnke, Wootton, Lehmann, Radke, Lucas, Nawas, Sibley and White2010). When tachyzoites enter the bradyzoite differentiation process, the cell cycle presents a long G2-phase during early events, and then mature bradyzoites stay arrested in G0 (Radke et al. Reference Radke, Guerini, Jerome and White2003). Therefore, replication and differentiation seem to be linked. Up to now, the cell signals responsible for modulating T. gondii replication are unknown. Moreover, it is still unknown whether there is a connection between host cell and tachyzoites in the modulation of the parasite replication process. The treatment of host cells with phosphatidylinositide 3-kinases (PI3K kinases) inhibitors such as LY294002 and wortmannin has been shown to reduce tachyzoite replication (Zhou et al. Reference Zhou, Quan, Lee, Shin and Cha2013). TgHsp90 PPI analysis has shown several putative kinases and phosphatases that could be involved in cell signalling (Table S1). However, the biological role of some of these proteins has not been assessed yet. We could infer that in the future some of these signalling kinases and phosphatases could be linked to Hsp90 and the cell cycle.

A clear association between Hsp90 and the cell cycle was observed early. This association involves cyclin-dependent kinase 1 CDK1 (also named cdc2 or cdc28), CDK2, CDK4, Cyclins B, D and E as well as the kinases Wee1, Myt-1, Plk1, Aurora B and survivin (Burrows et al. Reference Burrows, Zhang and Kamal2004). The inhibition of Hsp90 with XL888 blocks tumour cell progression and leads to cell cycle arrest and degradation of different client kinases such as CDK1 (cdc2/cdc28), ChK1 and Wee1 in vitro (Haarberg et al. Reference Haarberg, Paraiso, Wood, Rebecca, Sondak, Koomen and Smalley2013). Hsp90 is also linked to cyclin E degradation without binding to it (Bedin et al. Reference Bedin, Catelli, Cabanie, Gaben and Mester2009). Cyclin E regulates CDK2 and is required to initiate DNA replication at the G1/S transition. Although most of these cell cycle associated proteins are present in the T. gondii database, none of them were identified as a putative TgHsp90/p23 interactor. In addition, other proteins associated with the T. gondii replication process could be involved as TgHsp90 client proteins (e.g. SGT1-Skp1 pathway). Further analysis to elucidate cell cycle components that can be assisted by TgHsp90 should be performed.

STAGE DIFFERENTIATION

Toxoplasma gondii interconversion between rapidly growing tachyzoites and latent encysted bradyzoites involves coordinated, sequential, morphological and metabolic processes accompanied by gene expression events (Dzierszinski et al. Reference Dzierszinski, Nishi, Ouko and Roos2004). The TgHsp90 PPI network highlights the putative role of TgHsp90 in assisting glycolytic/respiration metabolism to obtain ATP, protein synthesis (which includes aminoacyl tRNA synthases), maturation of rRNAs, initiation and elongation factors and ribosomal proteins as well as protein degradation or processing (Table S1). These findings, obtained from T. gondii studies, have been further documented in other organisms (McClellan et al. Reference McClellan, Xia, Deutschbauer, Davis, Gerstein and Frydman2007; Pavithra et al. Reference Pavithra, Kumar and Tatu2007; Echeverria et al. Reference Echeverria, Bernthaler, Dupuis, Mayer and Picard2011). In addition, the PPI network of T. gondii p23 in tachyzoites and bradyzoites has also allowed the retrieval of these types of proteins as putative interactors (Echeverria et al. Reference Echeverria, Figueras, Vogler, Kriehuber, de Miguel, Deng, Dalmasso, Matthews, Matrajt, Haslbeck, Buchner and Angel2010). Noteworthy, in the bradyzoite stage, the parasite mitochondrion is down-regulated, whereas in the highly replicative tachyzoite, oxidative phosphorylation is essential for sufficient ATP generation (Bohne et al. Reference Bohne, Heesemann and Gross1994; Tomavo and Boothroyd, Reference Tomavo and Boothroyd1995). Toxoplasma gondii lactate dehydrogenase LDH1 is present in tachyzoites while LDH2 is found in bradyzoites, and the over-expression of LDHs in tachyzoites has been shown to enhance the bradyzoite conversion during stress (Liwak and Ananvoranich, Reference Liwak and Ananvoranich2009). For this reason, it is reasonable to find out that several enzymes associated to ATP generation would be regulated by TgHsp90 under differentiation conditions, a situation that generates stress in the tachyzoite.

Another important change during parasite differentiation is the protein repertoire in which some proteins should be replaced for another set of new proteins. In this regard, during bradyzoite development, the T. gondii eukaryotic initiation factor 2 alpha subunit (eIF2α) is phosphorylated, a PTM that restrains global protein synthesis, which persists in the bradyzoite stage (Narasimhan et al. Reference Narasimhan, Joyce, Naguleswaran, Smith, Livingston, Dixon, Coppens, Wek and Sullivan2008). Moreover, the inhibition of eIF2α dephosphorylation by salubrinal and guanabenz has been shown to reduce tachyzoite replication and induce bradyzoite formation, blocking its reactivation (Konrad et al. Reference Konrad, Queener, Wek and Sullivan2013). The TgHsp90 PPI network includes elongation factors and several ribosomal proteins that are involved in protein biosynthesis as putative interactors, but not the eIF2α kinase and eIF2α (Table S1). Even though these data link TgHsp90 with assisting the protein biosynthesis pathway, it remains to be seen if TgHsp90 could be regulating this key metabolic process during parasite differentiation.

One of the most interesting and curious aspects concerning TgHsp90 and parasite differentiation relies on protein translocation to the nucleus (Echeverria et al. Reference Echeverria, Matrajt, Harb, Zappia, Costas, Roos, Dubremetz and Angel2005). This could implicate a role in modulating transcription factors, chromatin and/or RNA processing factors. Although the Hsp90 PPI network presents some components of chromatin and transcriptional machinery as putative TgHsp90 interactors (Table S1), there is an important gap in this field since only a basal transcriptional machinery has been detected in apicomplexan parasites (Meissner and Soldati, Reference Meissner and Soldati2005). In addition, the involvement of an epigenetic mechanism in T. gondii gene regulation has been recently documented (Dalmasso et al. Reference Dalmasso, Sullivan and Angel2011; Croken et al. Reference Croken, Nardelli and Kim2012). On the other hand, the role of Hsp90 in transcriptional activities, chromatin modulation, RNA processing/splicing proteins and DNA replication/damage-response has been recently reviewed (Sawarkar et al. Reference Sawarkar, Sievers and Paro2012). Of note, a chromatin immunoprecipitation (ChIP) genome-wide analysis in Drosophila has shown that Hsp90 accumulates close to the transcription start sites of several protein-coding and miRNA-coding genes which are in a paused state of RNA polymerase II (Sawarkar et al. Reference Sawarkar, Sievers and Paro2012). Further studies should be carried out to determine the importance of nuclear translocation of TgHsp90 during bradyzoite development. These studies should consider not only a putative role in assisting nuclear proteins but also a role in gene expression and/or maintaining the paused state of RNA pol II.

HOST-CELL INVASION

Toxoplasma gondii presents a compartmental organization similar to other eukaryotes and a group of apicomplexan specific-secretory organelles (micronemes and rhoptries) located at the apical tip of the parasite, which are involved in attachment and invasion (Carruthers and Boothroyd, Reference Carruthers and Boothroyd2007; Sharma and Chitnis, Reference Sharma and Chitnis2013). The contents of micronemes and rhoptries are discharged at the time of invasion. Another important organelle, the inner membrane complex (IMC), together with the plasma membrane (PM), forms the pellicle, a triple lipid bilayer typical of apicomplexan parasites. The IMC is of critical importance for the parasite's cellular processes such as replication, gliding motility and invasion (Lourido et al. Reference Lourido, Tang and Sibley2012). Ahn et al. (Reference Ahn, Kim and Nam2003) have shown that inhibition of TgHsp90 blocks parasite invasion into the host cell. These authors have also shown that TgHsp90 is secreted during invasion and antibodies against this chaperone also block the host cell entry of the parasite. In addition to secreted Hsp90, the PPI network retrieved some proteins associated with cell adhesion and parasite invasion such as micronemes and rhoptry proteins (Table S1). Maybe, intracellular Hsp90 also participates in regulating the secretion and/or maturation of these proteins.