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Molecular detection of parasitic protozoa

Published online by Cambridge University Press:  01 November 1999

U. M. MORGAN  and
R. C. A. THOMPSON
U. M. MORGAN
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, 6150, Australia
R. C. A. THOMPSON
Affiliation:
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections and State Agricultural Biotechnology Centre, Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA, 6150, Australia
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Abstract

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The development of molecular diagnostic methods, particularly those utilizing PCR for the detection of parasitic protozoa will contribute greatly to the identification and control of these pathogens, by increasing speed of diagnosis, specificity and sensitivity, reproducibility and ease of interpretation. PCR methods are not without their problems however, and there is a need for laboratory procedures to be refined before PCR-based assays are accepted as the tools of choice for the routine detection of protozoan parasites. The application of PCR detection to various parasites is discussed.

Keywords

DetectionparasitesprotozoaPCRmicroscopyimmunodetection
Type
Current status
Information
Parasitology , Volume 117 , Issue 7 , November 1999 , pp. 73 - 85
Copyright
© 1999 Cambridge University Press