In the ablation zone of glacier habitats, cryoconite holes are known to harbor diverse microbial communities, including unique diatom floras distinct from those of surrounding aquatic and terrestrial systems. Besides descriptive studies, little is known about the diversity of cryoconite diatoms and their response to environmental stressors, particularly in low-latitude glaciers. This paper documents an extremely diversified diatom community in Antisana Glacier (Ecuador), reporting 278 taxa found in 54 surface holes, although with low individual abundances. Contrary to our expectations, assemblage structure did not respond to water physical or chemical characteristics, nor to cryoconite hole morphology, but to elevation. We demonstrate that elevation is a driver of diatom assemblages. Both alpha diversity (measured as Fisher's index) and species richness (corrected for unequal sample sizes) correlated negatively with elevation, suggesting a replacement toward simplified, poorer communities along this gradient. The taxonomic composition also changed significantly, as revealed by multivariate statistics. In summary, cryoconite holes are sites of high taxonomic diversity composed of taxa that are allochthonous in origin.

We compare different ways the bulk flow nonlinearity of glacier ice can be captured in an orthotropic rheology. Specifically, we compare the unapproximated orthotropic rheology, derived from plastic potential theory, to existing approximations that assume either the nonlinear viscosity or fluidity is identical to that of Glen's isotropic flow law. We find, overall, a reasonable agreement between the three orthotropic rheologies, and with existing Dye 3 ice-core deformation tests, although assuming Glen's viscosity provides the best approximation to the unapproximated rheology. Our results therefore suggest that previous studies based on either approximation to the orthotropic rheology are on relatively safe ground in the sense that both approximations generally agree with the unapproximated rheology and experimental data. Finally, we provide the forward and inverse analytical forms of all three rheologies for use in future numerical ice-flow modelling.