The present study was part of a larger surveillance effort to identify the determinants of African honey bee health, and, particularly, to detect honey bee pathogens across Kenya, where 160 colonies were examined from 32 apiaries (five colonies/apiary). From each colony, 20 individual foragers, nurse bees, worker pupae, and drone pupae were sampled separately. These were organized as 30 foragers, 32 nurse bees, 28 worker pupae, and 10 drone pupae pools. Nucleic acid was extracted from the pooled homogenates and tested using a panel of 18 different (RT-)PCR methods targeted at detecting Paenibacillus larvae, Melissococcus plutonius, Ascophaera apis, Aspergillus spp., Nosema ceranae, N. apis, Deformed wing virus (DWV), Varroa destructor virus 1 (VDV 1), Acute bee paralysis virus (ABPV), Sacbrood virus (SBV), Israeli acute paralysis virus (IAPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), and Kashmir bee virus. All amplified bands were sequenced and compared to the GenBank database. VDV 1 was the most abundant virus at 50% prevalence in the 100 bee pools. It was closely followed by DWV at 44%. The others were BQCV (36%), SBV (14%), IAPV (9%), ABPV (8%), and N. ceranae (5%). The pathogens co-existed within apiaries. VDV 1 was present in 66% of the apiaries, DWV in 69%, BQCV in 69%, SBV in 28%, IAPV in 22%, ABPV in 19%, and N. ceranae in 13%. The study concludes that these pathogens should be incorporated in honey bee disease surveillance activities in the region.

Dacus oleae (Gmelin) is a major pest of the olive tree Olea europea causing early fruit drop, ‘sting’ damage to table olives and substantial decreases in the quantity and quality of oil. This review describes the control strategies being developed and in current use—biological, trapping methods, sterile insect release, chemical insecticides, growth regulators and integrated control. Present problems and the implications of the controlling regimes on the disturbance of the agroecosystem are presented. Emphasis is given to newer methods which should be characterized by their specificity and effectivity.

Several Arthropod pests have been collected and identified on coffee, Coffea arabica. L. in Ethiopia, but only a few are economically important. Based on the plant part the pests feed on, they can be categorized as flower and flower bud feeders, leaf feeders, berry (fruit) feeders, twig and branch feeders borers and stem (trunk) borers.

Antestia, Antestiopsis intricata (Ghesquiere) Carayon and blotch leafminer, Leucoptera Caffeina (Washbourn) are the two most important pests feeding mainly on young fruits and leaves respectively.

Studies made regarding biology, feeding habits, population dynamics and natural enemies (parasitoids and predators) of Antestia are described. The buildup of this insect starts in March and reaches its peak around May/June although slight variations have been observed in various coffee growing areas. Young berries are the most preferred part of the plant. Antestia eggs 45–50% are attacked mainly by three species of parasistoids of which Asolcus suranus (Nixon) was found to be the most important.

The effect of infestation of lepidopteran borers on yield of maize was assessed at three locations in the forest and forest/savanna transition zones of Cameroon, during the first and second season of 1992. Maize was treated once or twice with the systemic carbofuran at different developmental stages. The species encountered were the stem-boring noctuids Busseola fusca and Sesamia calamistis, which oviposit on and attack pre-tasseling stages of maize; the stem-boring pyralid Eldana saccharina, which attacks plants at and after tasseling; and two ear borers, namely the pyralid Mussidia nigrivenella and the tortricid Cryptophlebia leucotreta. Generally, carbofuran reduced infestations by B. fusca and S. calamistis but had little or no effect on species attacking the plant's later stages. Thus early treatments with granules into the whorl were more efficient than late treatments as a side dressing, and a second late treatment had little effect. During the first season, late-attacking species were predominant and grain yield losses of 17–44 % were found in one location only. During the second season, species attacking young plants (mainly B. fusca) became predominant in all three locations and yield losses of 30 to 41 % were found in two of the three locations. The percentage of ears with damage was especially high during the second season. As green maize is an important source of cash income in the area and damaged ears cannot be sold, the economic loss was likely considerable.

In this study, the effect of exposing entomopathogenic bacteria isolated from macerated termite cadavers to varying intensities of a magnetic field for different periods of time on their pathogenic potential was examined; pathogenicity tests were carried out for each of the bacterial species. Two of the bacteria, Bacillus subtilis (Ehrenberg) Cohn and Serratia marcescens Bizio, were able to induce morbid effects on termites and both were re-isolated from the resulting cadavers. Reinfection using different concentrations of both bacteria was carried out on termites to determine the minimum lethal concentration required for pathogenicity. Bacillus subtilis was able to degenerate the termites at concentration values of 108 colony-forming units (cfu)/ml and S. marcescens at 107cfu/ml. Both bacteria were then exposed to magnetic fields of different intensities for different periods of time, after which they were used for reinfection of healthy termites. Post-infection study after the exposure of termites to magnetic field-treated bacterial cells revealed no reduction in the entomopathogenic potency of S. marcescens. As the extensive use of chemicals to control insect pests has been found to have detrimental effects on people and the environment, there is a pressing need to discover and develop new entomopathogens to control these insects biologically. Therefore, bacteria discovered in this study to have entomopathogenic potency against termites may be further studied and formulated into either powdery forms or suspensions to be applied to infested wood or wood products.

House flies (Musca domestica L.) are key pests of poultry and are managed worldwide with a variety of insecticides. However, extensive and injudicious use of insecticides has led to the development of resistance in many insect pests. Insecticide mixtures can increase the efficacy of the product and/or delay the development of resistance, thus making them a useful tool for pest control. In addition to their efficacy when used alone, mixtures of emamectin benzoate, spinosad or nitenpyram with either lambda-cyhalothrin or profenofos were assessed against a susceptible strain of house flies collected from poultry farms. The analysis revealed that combination indices for lambda-cyhalothrin/emamectin benzoate and lambda-cyhalothrin/spinosad mixtures were significantly >1, indicating an antagonistic effect. In contrast, the indices for all the tested ratios of lambda-cyhalothrin/nitenpyram were significantly < 1, suggesting a synergistic effect. For mixtures containing profenofos, combination indices varied significantly. For example, synergism was observed in a 1:1 mixture containing lambda-cyhalothrin and emamectin benzoate, but antagonism occurred when a higher proportion of the latter insecticide was used. Conversely, synergism in a mixture containing profenofos and nitenpyram was observed only when a lower proportion of the second insecticide was used. The present results suggest that ideal insecticide mixtures must be empirically determined and alternative strategies such as mosaics, rotations and cultural control should be considered for the management of the house fly.

Integration of the sterile insect technique (SIT) into the area-wide management of the peach fruit fly Bactrocera zonata (Saunders) is a promising alternative to the localized use of chemical control tactics. Implementation of the SIT requires adequate numbers of sterile male insects that are produced in large mass-rearing facilities. The present study was carried out to improve the rearing methods of the peach fruit fly so as to contribute to the development of effective mass-rearing protocols. Commercially available papaya, mango, peach and guava juices were found to be equally effective as oviposition stimulants. Moreover, with respect to the fecundity and fertility of flies, water was found to be as effective as the tested fruit juices. During the first 3 weeks, 90% of the total eggs produced were collected from adult oviposition cages holding 50,000 flies. A larval diet composed of sugarcane bagasse, ground maize, sugarcane sugar, waste brewer's yeast, wheat bran, benzoic acid, nipagin and water resulted in the following values for the quality control parameters: egg hatch 85%, pupal recovery >67%, pupal weight >4.2 g, pupation >95%, adult emergence >89%, and fliers >65%. Adult emergence and flight ability were similar for pupae placed in sand and vermiculite as the pupation medium.

The antibiotic effects of 12 different cotton genotypes on Bemisia tabaci (Gennadius) were studied during June–July and August–September 2003, with the objective to determine the biological parameters which are important to study the mechanism of antibiosis and to identify the genotypes in which antibiosis is operating. The detailed biology of whitefly, i.e. developmental period, survival, fecundity, longevity and sex ratio were studied under ‘no choice’ conditions, by confining the whiteflies in leaf cages. The study revealed that incubation period, nymphal developmental time, total developmental time, nymphal survival, total survival, fecundity and female longevity are the most important biological parameters for detecting the presence of antibiosis in resistant cotton genotypes against whiteflies. On the other hand, pupal period and survival, male longevity and sex ratio do not seem to play important roles in antibiosis. The combined effect of these parameters indicated that Gossypium arboreum genotype PA 183 was the most resistant while Bt 6304, TX Maroon 2–78 and F846 were the most susceptible ones. Other genotypes with moderate resistance include Supriya and LD 694.

Some factors affecting oviposition by Plodia interpunctella (Hubner) were studied under various controlled conditions in the laboratory. The effect of temperature, relative humidity, light and mating on oviposition were investigated.

Temperatures of 25 and 30°C were optimal for oviposition. The best condition was 30°C and 80% r.h. at which an average of 315.3 eggs were laid per female during its lifetime. The least favourable temperatures investigated were 15 and 35°C. Relative humidity did not have significant effect on the number of eggs laid at 15 and 25°C but at higher temperatures of 30 and 35°C, high r.h. increased the number of eggs laid by individual moths.

Light regimes of 12 hr light and 12hr darkness (LD) and 24 hr darkness (DD) were more favourable than 24 hr light (LL) for oviposition.

Virgin females laid fewer eggs than mated females while prolonged virginity reduced the number of eggs laid and caused resorption of immature oocytes and retention of ripe eggs in the ovaries.

Food losses caused by insects during postharvest storage are of paramount economic importance worldwide, especially in Africa. Laboratory bioassays were conducted in stored grains to determine the toxicity of powders of Eugenia aromatica and Moringa oleifera alone or combined with enhanced diatomaceous earth (Probe-A® DE, 89.0% SiO2 and 5% silica aerogel) to adult Sitophilus granarius, Tribolium castaneum and Acanthoscelides obtectus. Adult mortality was observed up to 7 days, while progeny production was recorded at 6–10 weeks. LD50 and LT50 values for adult test insects exposed to plant powders and DE, showed that A. obtectus was the most susceptible towards the botanicals (LD50 0.179% and 0.088% wt/wt for E. aromatica and M. oleifera, respectively), followed by S. granarius. Tribolium castaneum was most tolerant (LD50 1.42% wt/wt and 1.40% wt/wt for E. aromatica and M. oleifera, respectively). The combined mixture of plant powders and DE controlled the beetles faster compared to the plant powders alone. LT50 ranged from 55.7 h to 62.5 h for T. castaneum exposed to 1.0% M. oleifera and 1.0% DE, and 0.5% E. aromatica and 1.0% DE, respectively. Botanicals caused significant reduction of F1 adults compared to the control. Combined action of botanical insecticides with DE as a grain protectant in an integrated pest management approach is discussed.

This study was conducted in the Knuckles Forest Region in central Sri Lanka, and investigated how termite species richness, abundance and functional group diversity vary in different montane forest types and identified the likely causes of this pattern. Termite diversity declined with increased elevation, with upper montane forests recording a single endemic species, Postelectrotermes militaris Desneux. Transect sampling in lower montane forests yielded 26 species, with a higher number from dry forests (22 species) than from wet forests (15 species). Species specificity also was high in dry forests (11 species) compared with wet forests (four species). Termite abundance did not show a distinct trend in dry and wet forests. Live-wood termites were present only in upper montane and high-altitude lower montane dry forests. Wet forests had a higher relative abundance (78%) but not species richness (40%) of soil and soil–wood interface feeders. In dry forests, both species richness (82%) and abundance (88%) of fungus-growing wood feeders were higher. The study suggests that key drivers of the species distribution pattern are low temperature and differing forest floor conditions. In the upper montane forest floor where earthworms dominate, wet soil and damp, woody litter riddled with beetles are not favourable for termites. In lower montane wet forests, moist, thick decomposing leaf litter and in dry forests, drier, relatively undecomposed leaf litter with many dry sticks and branches support species with specific food habits.

The anthocorid bug Orius albidipennis Reuter is a generalist predator that feeds on the whitefly Bemisia tabaci Gennadius and the strawberry spider mite Tetranychus turkestani Ugarov & Nikolski in greenhouse crops. There are no previous studies on the potential efficacy of the predatory bug against these pests on greenhouse crops. We report on the efficacy and the prey preference of the predator to control these pests on different host plants under laboratory conditions. In a laboratory experiment, we estimated the predation rates of O. albidipennis at different densities of each prey after 24 h on cucumber and sweet pepper leaves. Predation rates of the predatory bug to T. turkestani and B. tabaci were significantly higher on sweet pepper leaf than on cucumber leaf. We studied the effect of plant species on prey preference and switching of O. albidipennis to B. tabaci and T. turkestani using Manly's α index values and Murdoch's no-switch line, respectively. Our results show that O. albidipennis prefers T. turkestani to B. tabaci on both host plants but its preference for T. turkestani on sweet pepper is significantly greater than on cucumber. Moreover, on sweet pepper, preference values are completely fitted by Murdoch's no-switch line. The findings suggest that morphological defence traits of plants, such as hairy leaves of cucumber, may effectively change prey preference and reduce predation success of O. albidipennis.

A survey involving more than 129 farmers was carried out in 1998 and 1999 in Ouémé, Benin, to investigate the importance of pests and diseases as constraints to cowpea (Vigna unguiculata (L.) Walp.) production. Results indicated that in the Ouémé valley Sclerotium rolfsii Sacc. and Aphis craccivora Koch are specific concerns. Weeds such as justicia anselliana (Nees) T. Anders, Ipomoea aquatica Forsskal and Commelina erecta L. were also reported as being troublesome. On the Ouémé plateau Imperata cylindrica L. Beauv. was reported to be a major weed. Callosobruchus maculatus Fab. and Bruchidius atrolineatus Fab. may cause up to 100% loss within a few months in storage. Birds and rodents were also reported. In certain areas on the plateau, farmers have developed pest control methods based on indigenous knowledge. In the valley the population density of Eichhornia crassipes (Mart) Solms. during flooding is used by farmers to predict aphid infestation. A number of plant species used to protect cowpea were reported. In Gbékandji village, natural enemies such as Rhabdepyris sp., Evania sp., and Chelonus sp., were observed. They were rare in the valley, where farmers rely on chemical control. Farmers reported alternative hosts of cowpea pests from the families Fabaceae (eight species), Mimosaceae (two species), Ceasalpiniaceae (five species), Capparidaceae (two species) and Pontederiaceae (one species). Based on the hierarchy of constraints, sustainable integrated pest management technologies are being developed with farmers using a participatory approach.

Sources of information on the biology and ecology of Chilo species are reviewed on a world-wide basis, with emphasis on the major pest species which are stem borers of rice, maize, sugar-cane, sorghum and other cereal crops and grasses. Information on geographical distribution, host plants, general biology, ecology and population dynamics is summarized for the main species, especially for C. partellus (Swinhoe), C. suppressalis (Walker), C. infuscatellus (Snellen), C. sacchariphagus (Bojer), C. auricilius (Dudgeon), C. zacconius Bleszynski and C. agamemnon Bleszynski. Other species are also noted, including C. diffusilineus (de Joannis), C. panici Wang and Sung, C. terrenellus Pagenstecher, C. polychrysus (Meyrick), C. plejadellus Zincken, C. niponella (Thunberg) and C. orichalcociliellus (Strand). Requirements for additional and better organized information are identified.

Aulacophora foveicollis Lucas causes economic losses to creeping cucumber [Solena amplexicaulis (Lam.) Gandhi] growers in India and Bangladesh because adults feed on the leaves and flowers causing death of the plant. The insect is a generalist herbivore as it also causes damage to pumpkin, bottle gourd, sponge-gourd and gac fruit production by feeding on leaves and flowers of these plants. At present, insects are controlled with insecticides, which are harmful to human health and the environment. We studied the behavioural responses of adult A. foveicollis to flower surface waxes and synthetic compounds mimicking flower surface waxes to determine their potential for monitoring this pest. The gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID) analyses of S. amplexicaulis flower (50 g) surface waxes indicated presence of 17.9 and 3.1 mg alkanes and free fatty acids, respectively. Seventeen n-alkanes from n-C15 to n-C34 and 16 free fatty acids from C10:0 to C22:0 were detected in the flower surface waxes. Heptacosane was predominant among n-alkanes representing 2748.1 µg; whereas, pentadecanoic acid was the major fatty acid accounting for 466.6 µg. Aulacophora foveicollis were attracted to the flower surface waxes at concentrations of 4 to 8 μg/ml, as demonstrated by a Y-tube olfactometer bioassay. Using a dose response bioassay, the insect was shown to be attracted to individual synthetic pentadecane, heptacosane, nonacosane, undecanoic acid and nonadecanoic acid at 0.70, 0.70, 1.20, 1.60 and 1.40 µg/ml, respectively. The insect displayed highest attraction to a synthetic mixture of 0.70, 1.23, 0.77, 0.84, 0.94 and 0.74 µg/ml of pentadecane, heptacosane, nonacosane, undecanoic acid, lauric acid and nonadecanoic acid, respectively, and hence, this combination might be used for insect pest management such as in baited traps.

The potential egg production and egg viability of the potato tuber moth, Phthorimaea operculella (Zell.) was significantly lowered when moths of either sex were exposed in 250 ml glass jars to vapours of orange peel oil emanating from 160 μ oil. Oviposition and egg hatching was totally inhibited when female moths of both sexes were exposed to 220 μl of the oil.

A study was conducted to investigate whether a lack of pollinators, pollination and therefore, a shortage of fruit set of perennial plants is more likely to occur in overgrazed land than farmland without grazing pressure, as this could have considerable effects on the reproduction of certain plant species. The study was conducted at two adjacent BIOTA biodiversity observatories (<url>www.biota-africa.org</url>) in Namaqualand, South Africa. Individuals of certain plant species of the family of Aizoaceae were measured and flower and fruit production recorded. Malaise traps and transect walks were used to document insect diversity. Preliminary results indicate that not all plant species show reduced flower production but that fruit set is indeed compromised on overgrazed land. Insect diversity and abundance is partially reduced under heavy grazing pressure, especially in locations where the vegetation cover is low and dominated by unpalatable plant species.

Susceptibility studies keep farmers informed, and enhance breeding programmes' testing against infestation and damage by stored product pests. Therefore, the susceptibility of 20 elite maize varieties to Sitophilus zeamais infestation was investigated in the laboratory at 32.7 °C ambient temperature and 70.4% relative humidity. The varieties tested were obtained from the Maize Breeding Units of International Institute of Tropical Agriculture (IITA), and Institute of Agricultural Research and Training (IAR&T), both in Ibadan, Nigeria. Susceptibility was assessed, using as indices, weevil mortality and survival rates, percentage grain damage, percentage grain weight loss, weight of grain powder, weevil development time, and number of emerged adults. The susceptibility index was determined post-infestation at 56 days using Dobie's formula and at 90 days from a new formula derived from Dobie's formula. Susceptible varieties produced significantly (P≤ 0.05) high numbers of F1 and F2 progeny, and had significantly (P≤ 0.05) low weevil mortality and early first weevil emergence. Grain damage and loss increased with storage duration. There was no visible damage to varieties 2000SYNEE-WSTR and TZBRELD3C5 at 56 days post-infestation. At 90 days, the two varieties had the lowest percentage grain damage, percentage weight loss, and weight of grain powder. The index of susceptibility at F2 progeny showed that the resistant varieties decreased in number from 17 (85%) to 13 (65%) and the moderately resistant varieties increased from 2 (10%) to 6 (30%). This suggests the effect of prolonged storage duration on the inherent ability of varieties to resist weevil attack. Variety PVASYN3F2 was susceptible. The factors conferring high resistance to 2000SYNEE-WSTR and TZBRELD3C5 should be investigated. They should be promoted to control S. zeamais infestation and damage in stored maize.

The effects of methanol extract of Peganum harmala L. seeds were evaluated against Tribolium castaneum (Herbst). Larval growth was inhibited and food intake reduced when the extract was incorporated into the diet at concentrations of 2.5, 5 and 10%. All doses caused an increase in the duration of larval period and completely inhibited F1 progeny production. The main body metabolites were determined in last-instar larvae after feeding for 8 days on the diet treated with different doses. Protein and lipid contents were decreased with concentrations >2.5%. Glycogen content was reduced at a concentration of 10%. Tribolium castaneum larvae possessed two major and one minor α-amylase isoforms as determined by gel assays. Larvae fed the treated diet presented less activity of the major isoforms, while activity of the minor isoform was totally inhibited. The activity of this enzyme is not inhibited in vitro by seed extracts. Histological studies showed that P. harmala extracts caused a severe cytotoxicity on the epithelial cells of the midgut resulting in marked vacuolization of the cytoplasm and a large intercellular space. These findings suggest that seed extracts of P. harmala exert their toxic effect by severely damaging the midgut epithelial cells. This naturally occurring plant extract could be useful for control of T. castaneum.

Bruchid beetles cause high losses of stored legumes in the tropics. Chemical control is inappropriate for the small scale farmer and the use of alternative measures should be encouraged. Different biological methods of control such as cultural, physical, varietal, biological, biorational and genetic control are reviewed. Cultural control techniques such as timely and frequent harvesting, legumes planted away from granaries, crop hygiene and storage in pods, are within the technical and financial means of the small scale farmer. The use of inert substances, vegetable oils and repellent plants may be cheap and effective if properly managed. The use of controlled atmosphere, vacuum, heating, cooling and sterilizing, requires relatively advanced techniques. Growth regulators and pheromones are currently not available for control. Varieties resistant to bruchids have been identified, but incorporation of resistance into suitable varieties has proven to be difficult. Biological control of bruchids should receive more attention in particular research on the introduction and conservation of natural enemies. To effectively implement control measures at farmer's level socio-economic and cultural aspects should be taken into account. Proven appropriate techniques should be combined in an overall integrated pest management strategy.