Higher vitamin D concentrations have been proposed as a protective ‘seasonal stimulus’ against influenza, and there are suggestions for associations with other aspects of respiratory health. The aim of the present study was to investigate the relationship of current vitamin D status (measured by 25-hydroxyvitamin D, 25(OH)D) with respiratory infections and lung function. We used cross-sectional data from 6789 participants in the nationwide 1958 British birth cohort who had measurements of 25(OH)D, lung function (forced expiratory volume in 1 s (FEV1) and forced vital capacity (FVC)) and respiratory infections available from the age of 45 years. In this population, the prevalence of respiratory infections had a strong seasonal pattern in the opposite direction to the pattern for 25(OH)D concentrations. Each 10 nmol/l increase in 25(OH)D was associated with a 7 % lower risk of infection (95 % CI 3, 11 %) after adjustment for adiposity, lifestyle and socio-economic factors. For FEV1 and FVC, each 10 nmol/l increase in 25(OH)D was associated with 8 (95 % CI 3, 13) ml and 13 (95 % CI 7, 20) ml higher volume, respectively, after controlling for covariates. Associations of 25(OH)D with FEV1 and FVC were only slightly attenuated after further adjustment for infection and other respiratory illness. In conclusion, vitamin D status had a linear relationship with respiratory infections and lung function. Randomised controlled trials are warranted to investigate the role of vitamin D supplementation on respiratory health and to establish the underlying mechanisms.

Rodents respond to a chronic high-fat diet (HFD) in two ways: some readily become obese (obesity prone, OP) and others do not (obesity resistant, OR). Although several hypotheses have been proposed, the mechanisms underlying the inter-individual susceptibility to diet-induced obesity remain to be fully defined. In the present study, two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption ionisation time-of-flight MS was carried out for identification of differentially expressed liver proteins in OP and OR rats fed a HFD, in an attempt to discover marker proteins involved in susceptibility and/or resistance to obesity in rat liver. The 2-DE analysis demonstrated that forty spots from 380 visualised spots were differentially regulated between the groups. Among these forty spots, twelve were differentially expressed proteins between OP and OR rats, reaching statistical significance. Of these, five proteins have already been linked to obesity; however, seven proteins involved in obesity susceptibility or resistance were identified for the first time in the present study. In order to validate the proteomic results and gain insight into the metabolic changes between the OP and OR groups, we further confirmed the expression pattern of some proteins of interest by Western blot analysis. Combined results of proteomic analysis with Western blot analysis revealed that reduced lipogenesis and increased fat oxidation were achieved in the livers of OR rats. In conclusion, the present proteomic study is an important advance over the previous steps required for identification of OP and OR rats, and should prove valuable in the search for the pathogenesis of obesity in humans.

The effect of monosodium glutamate (MSG) supplementation in soup or broth on satiety is not well understood. In the present study, the relative effects of four chicken broths with or without added MSG on motivational ratings and energy intakes at the next meal were compared using a double-blinded, within-subject design. A total of thirty-five normal-weight women, aged 20–40 years, took part in four study sessions. The four broths were base chicken broth (63 kJ), broth with added MSG (1·19 g) and nucleotides (0·03 g), broth with added MSG (1·22 g), and broth with added fat (BAF; 681 kJ). The preloads were presented twice at 09.00 and 11.15 hours for a maximum cumulative dose of 2·44 g MSG. Motivational ratings were collected before and at 15 min intervals post-ingestion for a total of 210 min. A test lunch meal was served at 12.00 hours, and plate waste was measured. The addition of MSG to chicken broth did not increase energy intakes at lunch or affect motivational ratings over the entire testing session. Both hunger and desire to snack between the second preload exposure and the test meal were significantly reduced in the MSG condition relative to the base broth condition (both, P =  0·03). However, only the BAF significantly suppressed energy intakes at lunch compared with the base broth control condition. Supplementing chicken broth with MSG can increase subjective ratings for satiety but does not alter energy intake at the next meal relative to an equal energy broth without added MSG.

The present study examines the prevalence and characteristics of vegetarians in the Taiwanese elderly. We analysed data from the Elderly Nutrition and Health Survey in Taiwan (1999–2000), which used a multi-staged, stratified, clustered probability sampling design. Community-dwelling elderly (n 1071), aged 65 years and older, were included. Sociodemographic, anthropometric, lifestyle and metabolic variables, and eating habits were obtained through household interviews and health examinations. Nutrient intake was assessed using a 24 h dietary recall. Multivariable logistic regression analysis was performed to examine factors significantly and independently associated with vegetarian status and to estimate the OR of the hypertension and the metabolic syndrome (MS) for vegetarians compared with omnivores. About 25 % of the Taiwanese elderly persons were vegetarians. Systolic pressure (OR 1·01, 95 % CI 1, 1·02, P = 0·038), female sex (OR 5·02, 95 % CI 3·11, 8·1, P < 0·001), smoking status (P = 0·034; current smoker (OR 0·45, 95 % CI 0·24, 0·85, P = 0·014)) and regular exercise (OR 1·87, 95 % CI 1·37, 2·56, P < 0·001) were independently associated with vegetarian status among Taiwanese elderly persons. Vegetarians consumed significantly lower daily total energy (P < 0·001), lower cholesterol (P = 0·002), a higher percentage of fat as PUFA (P = 0·022), higher Ca (P < 0·001) and higher crude fibres (P = 0·041) compared with omnivores. Between the two vegetarian diets, ovo- or lacto-vegetarian diets contained more beneficial micronutrients, such as K, Ca and Mg, and higher crude fibres than vegan diets (all P < 0·05). The likelihood of having hypertension and the MS was not significantly different between vegetarians and omnivores as examined by sex- or multivariate-adjusted logistic regression.

A total of 539 pure-bred and seventy-five cats without a pedigree were weighed and scored at cat shows or in veterinary surgeries. Data from normal-weight cats with a body condition score (BCS) of 5 (ideal) were only used. Breeds were grouped into five classes. For female cats, the mean weight for these groups were as follows: very light (2·8 kg); light (3·2 kg); medium (3·5 kg); large (4·0 kg); giant (4·9) kg. For male cats, the corresponding values were 3·6, 4·2, 4·3, 5·1 and 6·1 kg. Siamese/Oriental Shorthair were identified as a very light breed, the Norwegian Forest and the Siberian Cat as a large breed and the Maine Coon as a giant breed. Males and females of the same breed did not always belong to the same class. In some breeds, individuals of the same sex were found in two different classes. The percentage of intact overweight cats (BCS >5) was low (7 % of intact males, 3 % of intact females). Incidence of overweight in neutered cats was 50 % in males and 38 % in females. Among pedigreed cats, there were differences in the incidence of overweight in neutered cats: high in Norwegian Forest Cats (males 75 %, females 50 %) and low in Siamese/Oriental Shorthair Cats (males 25 %, females 1 %). Cats with a BCS of 6, 7 and 8 had on average 120, 154 and 214 % of the normal weight of their breed, respectively.

The intestinal absorption of tocotrienols (TCT) in dogs is, to our knowledge, so far unknown. Adult Beagle dogs (n 8) were administered a single oral dosage of a TCT-rich fraction (TRF; 40 mg/kg body weight) containing 32 % α-TCT, 2 % β-TCT, 27 % γ-TCT, 14 % δ-TCT and 25 % α-tocopherol (α-TCP). Blood was sampled at baseline (fasted), 1, 2, 3, 4, 5, 6, 8 and 12 h after supplementation. Plasma and chylomicron concentrations of TCT and α-TCP were measured at each time point. Plasma TAG were measured enzymatically, and plasma antioxidant capacity was assessed by the Trolox equivalent antioxidant capacity assay. In fasted dogs, levels of TCT were 0·07 (sd 0·03) μmol/l. Following the administration of the TRF, total plasma TCT peaked at 2 h (7·16 (sd 3·88) μmol/l; P < 0·01) and remained above baseline levels (0·67 (sd 0·44) μmol/l; P < 0·01) at 12 h. The TCT response in chylomicrons paralleled the increase in TCT in plasma with a maximum peak (3·49 (sd 2·06) μmol/l; P < 0·01) at 2 h post-dosage. α-TCP was the major vitamin E detected in plasma and unaffected by TRF supplementation. The Trolox equivalent values increased from 2 h (776 (sd 51·2) μmol/l) to a maximum at 12 h (1130 (sd 7·72) μmol/l; P < 0·01). The results show that TCT are detected in postprandial plasma of dogs. The increase in antioxidant capacity suggests a potential beneficial role of TCT supplementation in the prevention or treatment of several diseases in dogs.