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Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites

Published online by Cambridge University Press:  22 February 2005

A. HUDSON  and
J. T. ELLIS
A. HUDSON
Affiliation:
Department of Cell and Molecular Biology, University of Technology, Sydney, St Leonard's Campus, Westbourne Street, Gore Hill, NSW 2065, Australia
J. T. ELLIS
Affiliation:
Department of Cell and Molecular Biology, University of Technology, Sydney, St Leonard's Campus, Westbourne Street, Gore Hill, NSW 2065, Australia
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Abstract

Mycoplasmas are common contaminants of eukaryotic cells grown in tissue culture. A commercially available Mycoplasma Removal Agent (MRA) was therefore assessed for its effect on tachyzoites of Neospora caninum, in order to determine its suitability for further use in parasite cell cultures. Analyses of tachyzoite and excreted-secreted proteins and antigens by SDS-PAGE and Western blotting show that MRA treatment results in the rapid selection of a mutant population that differs from the control and parental lines in its protein and antigen content. The treatment of N. caninum cultures with MRA is therefore not recommended for the eradication of Mycoplasma.

Keywords

Neospora caninumMycoplasmaantibioticquinoloneculture
Type
Research Article
Information
Parasitology , Volume 130 , Issue 6 , June 2005 , pp. 607 - 610
Copyright
© 2005 Cambridge University Press

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References

REFERENCES

BEBEAR, C. M., GRAU, O., CHARRON, A., RENAUDIN, H., GRUSON, D. & BEBEAR, C. ( 2000). Cloning and nucleotide sequence of the DNA gyrase (gyrA) gene from Mycoplasma hominis and characterization of quinolone-resistant mutants selected in vitro with trovafloxacin. Antimicrobial Agents and Chemotherapy 44, 2719–2727.CrossRefGoogle Scholar
BEBEAR, C. M., RENAUDIN, H., CHARRON, A., CLERC, M., PEREYRE, S. & BEBEAR, C. ( 2003). DNA gyrase and topoisomerase IV mutations in clinical isolates of Ureaplasma spp. and Mycoplasma hominis resistant to fluoroquinolones. Antimicrobial Agents and Chemotherapy 47, 3323–3325.CrossRefGoogle Scholar
FICHERA, M. E. & ROOS, D. S. ( 1997). A plastid organelle as a drug target in apicomplexan parasites. Nature, London 390, 407–409.CrossRefGoogle Scholar
HENSMANN, M. & KWIATKOWSKI, D. ( 2001). Cellular basis of early cytokine response to Plasmodium falciparum. Infection and Immunity 69, 2364–2371.CrossRefGoogle Scholar
KHAN, A. A., ARAUJO, F. G., BRIGHTY, K. E., GOOTZ, T. D. & REMINGTON, J. S. ( 1999). Anti-Toxoplasma gondii activities and structure-activity relationships of novel fluoroquinolones related to trovafloxacin. Antimicrobial Agents and Chemotherapy 43, 1783–1787.Google Scholar
KHAN, A. A., SLIFER, T., ARAUJO, F. G. & REMINGTON, J. S. ( 1996). Trovafloxacin is active against Toxoplasma gondii. Antimicrobial Agents and Chemotherapy 40, 1855–1859.Google Scholar
MILLER, C. M. D., QUINN, H. E., WINDSOR, P. A. W. & ELLIS, J. T. ( 2002). Characterisation of the first Australian isolate of bovine Neospora caninum. Australian Veterinary Journal 80, 620–625.CrossRefGoogle Scholar
MISAWA, S., AOSHIMA, M., TAKAKU, H., MATSUMOTO, M. & HAYASHI, H. ( 1994). High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme. Journal of Biotechnology 36, 145–155.CrossRefGoogle Scholar
NAKAI, N., KAWAGUCHI, C., NAWA, K., KOBAYASHI, S., KATSUTA, Y. & WATANABE, M. ( 2000). Detection and elimination of contaminating microorganisms in transplantable tumors and cell lines. Experimental Animals 49, 309–313.CrossRefGoogle Scholar
RAPPELLI, P., CARTA, F., DELOGU, G., ADDIS, M. F., DESSI, D., CAPPUCCINELLI, P. & FIORI, P. L. ( 2001). Mycoplasma hominis and Trichomonas vaginalis symbiosis: multiplicity of infection and transmissibility of M. hominis to human cells. Archives of Microbiology 175, 70–74.Google Scholar
ROWE, J. A., SCRAGG, I. G., KWIATKOWSKI, D., FERGUSON, D. J., CARUCCI, D. J. & NEWBOLD, C. I. ( 1998). Implications of Mycoplasma contamination in Plasmodium falciparum cultures and methods for its detection and eradication. Molecular and Biochemical Parasitology 92, 177–180.CrossRefGoogle Scholar
TURRINI, F., GIRIBALDI, G., VALENTE, E. & ARESE, P. ( 1997). Mycoplasma contamination of Plasmodium cultures – A case of parasite parasitism. Parasitology Today 1997, 367–368.CrossRefGoogle Scholar
UPHOFF, C. C. & DREXLER, H. G. ( 2002). Comparative antibiotic eradication of Mycoplasma infections from continuous cell lines. In Vitro Cellular and Developmental Biology. Animal 38, 86–89.2.0.CO;2>CrossRefGoogle Scholar
UPHOFF, C. C. & DREXLER, H. G. ( 2004). Elimination of Mycoplasma from infected cell lines using antibiotics. Methods in Molecular Medicine 88, 327–334.Google Scholar