There is increasing use of functional imaging data to understand the macro-connectome of the human brain. Of particular interest is the structure and function of intrinsic networks (regions exhibiting temporally coherent activity both at rest and while a task is being performed), which account for a significant portion of the variance in functional MRI data. While networks are typically estimated based on the temporal similarity between regions (based on temporal correlation, clustering methods, or independent component analysis [ICA]), some recent work has suggested that these intrinsic networks can be extracted from the inter-subject covariation among highly distilled features, such as amplitude maps reflecting regions modulated by a task or even coordinates extracted from large meta analytic studies. In this paper our goal was to explicitly compare the networks obtained from a first-level ICA (ICA on the spatio-temporal functional magnetic resonance imaging (fMRI) data) to those from a second-level ICA (i.e., ICA on computed features rather than on the first-level fMRI data). Convergent results from simulations, task-fMRI data, and rest-fMRI data show that the second-level analysis is slightly noisier than the first-level analysis but yields strikingly similar patterns of intrinsic networks (spatial correlations as high as 0.85 for task data and 0.65 for rest data, well above the empirical null) and also preserves the relationship of these networks with other variables such as age (for example, default mode network regions tended to show decreased low frequency power for first-level analyses and decreased loading parameters for second-level analyses). In addition, the best-estimated second-level results are those which are the most strongly reflected in the input feature. In summary, the use of feature-based ICA appears to be a valid tool for extracting intrinsic networks. We believe it will become a useful and important approach in the study of the macro-connectome, particularly in the context of data fusion.

The Maghreb (north-west Africa) played an important role during the Palaeolithic and later in connecting the western Mediterranean from the Phoenician to Islamic periods. Yet, knowledge of its later prehistory is limited, particularly between c. 4000 and 1000 BC. Here, the authors present the first results of investigations at Oued Beht, Morocco, revealing a hitherto unknown farming society dated to c. 3400–2900 BC. This is currently the earliest and largest agricultural complex in Africa beyond the Nile corridor. Pottery and lithics, together with numerous pits, point to a community that brings the Maghreb into dialogue with contemporaneous wider western Mediterranean developments.

Aquatic ecosystems - lakes, ponds and streams - are hotspots of biodiversity in the cold and arid environment of Continental Antarctica. Environmental change is expected to increasingly alter Antarctic aquatic ecosystems and modify the physical characteristics and interactions within the habitats that they support. Here, we describe physical and biological features of the peripheral ‘moat’ of a closed-basin Antarctic lake. These moats mediate connectivity amongst streams, lake and soils. We highlight the cyclical moat transition from a frozen winter state to an active open-water summer system, through refreeze as winter returns. Summer melting begins at the lakebed, initially creating an ice-constrained lens of liquid water in November, which swiftly progresses upwards, creating open water in December. Conversely, freezing progresses slowly from the water surface downwards, with water at 1 m bottom depth remaining liquid until May. Moats support productive, diverse benthic communities that are taxonomically distinct from those under the adjacent permanent lake ice. We show how ion ratios suggest that summer exchange occurs amongst moats, streams, soils and sub-ice lake water, perhaps facilitated by within-moat density-driven convection. Moats occupy a small but dynamic area of lake habitat, are disproportionately affected by recent lake-level rises and may thus be particularly vulnerable to hydrological change.

The expansion of transportation and service corridors has numerous, well-documented adverse effects on wildlife. However, little research on this topic has been translated into mitigating the effects of habitat fragmentation caused by road development on primates. The establishment of canopy bridges has proven to be an effective conservation intervention. Of the completed primate canopy bridge projects reported in the literature, to our knowledge, all attempt to mitigate the impacts caused by singular, linear infrastructure routes. Here we provide recommendations for the establishment of a network of natural and artificial canopy bridges over roads throughout Langkawi Island, Malaysia, to reduce rates of roadkill and support the movement of primates and other arboreal animals across the island by identifying suitable sites and appropriate tree species to be planted (including Ficus racemosa and Ficus fistulosa), bridge materials and post-installation monitoring. The establishment of this pioneering trans-island canopy bridge network could function as a model to enhance connectivity for arboreal animals in other important wildlife habitat sites in Malaysia and beyond that are affected by fragmentation from linear infrastructure. We have begun discussions with relevant authorities, partners and other pertinent parties, focusing on the initiation of construction of the canopy bridge network in 2024.

Hedeby was the largest town in the Viking North. Investigations have identified imports at the site from central and northern Scandinavia revealing long-distance connections. The chronology of this trade, however, is unclear. Here, the authors use a typological-biomolecular approach to examine connections during the early Viking Age. The application of ZooMS to an assemblage of antler combs, stylistically dated to the ninth century AD, reveals nearly all were made of reindeer antler. As most craft production waste from Hedeby comprises red deer antler, it is argued that these combs were manufactured elsewhere, perhaps hundreds of kilometres further north. The results have implications for understanding of production and regional connectivity in early medieval Scandinavia.

Many organisms live in fragmented populations, which has profound consequences on the dynamics of associated parasites. Metapopulation theory offers a canonical framework for predicting the effects of fragmentation on spatiotemporal host–parasite dynamics. However, empirical studies of parasites in classical metapopulations remain rare, particularly for vector-borne parasites. Here, we quantify spatiotemporal patterns and possible drivers of infection probability for several ectoparasites (fleas, Ixodes trianguliceps and Ixodes ricinus) and vector-borne microparasites (Babesia microti, Bartonella spp., Hepatozoon spp.) in a classically functioning metapopulation of water vole hosts. Results suggest that the relative importance of vector or host dynamics on microparasite infection probabilities is related to parasite life-histories. Bartonella, a microparasite with a fast life-history, was positively associated with both host and vector abundances at several spatial and temporal scales. In contrast, B. microti, a tick-borne parasite with a slow life-history, was only associated with vector dynamics. Further, we provide evidence that life-history shaped parasite dynamics, including occupancy and colonization rates, in the metapopulation. Lastly, our findings were consistent with the hypothesis that landscape connectivity was determined by distance-based dispersal of the focal hosts. We provide essential empirical evidence that contributes to the development of a comprehensive theory of metapopulation processes of vector-borne parasites.

Coastal marine environments are subject to a variety of anthropogenic pressures that can negatively impact habitats and the biodiversity they harbor. Conservation actions such as marine protected areas, marine reserves, and other effective area-based conservation measures, are pivotal tools for protecting coastal biodiversity. However, to be effective, conservation area networks must be planned through a systematic conservation planning (SCP) approach. Recently, such approaches have begun to orient their goals toward the conservation of different biodiversity facets and to integrate different types of data. In this review, we illustrate how genetic data and molecular techniques can bring useful knowledge for SCP approaches that are both more comprehensive (sampling the full range of biodiversity) and more adequate (ensuring the long-term persistence of biodiversity). With an emphasis on coastal organisms and habitats, we focus on phylogenetic analysis, the estimation of neutral and adaptive intraspecific genetic diversity at different spatial levels (alpha, beta, and gamma), the study of connectivity and dispersal, and the information obtainable from environmental DNA techniques. For each of these applications, we discuss the benefits of its integration into SCP for coastal systems, its strengths and weaknesses, and the aspects yet to be developed.