The genus Berberis (Berberidaceae) in Nepal is revised and 21 species are recognised. Two species, Berberis pendryi Bh.Adhikari and Berberis karnaliensis Bh.Adhikari, are newly described and 11 taxa are lectotypified. A key to species is provided and all species are fully described and illustrated, and their distributions within Nepal mapped. An IUCN conservation assessment is given for each species.