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Genotype and subtype analysis of Cryptosporidium isolates from calves and lambs in Galicia (NW Spain)

Published online by Cambridge University Press:  12 April 2010

PABLO DÍAZ ,
JOAQUÍN QUÍLEZ ,
RACHEL M. CHALMERS ,
ROSARIO PANADERO ,
CEFERINO LÓPEZ ,
CARIDAD SÁNCHEZ-ACEDO ,
PATROCINIO MORRONDO  and
PABLO DÍEZ-BAÑOS
PABLO DÍAZ*
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain
JOAQUÍN QUÍLEZ
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
RACHEL M. CHALMERS
Affiliation:
UK Cryptosporidium Reference Unit, NPHS Microbiology Swansea, Singleton Hospital, Swansea SA2 8QA, UK
ROSARIO PANADERO
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain
CEFERINO LÓPEZ
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain
CARIDAD SÁNCHEZ-ACEDO
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain
PATROCINIO MORRONDO
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain
PABLO DÍEZ-BAÑOS
Affiliation:
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain
*
*Corresponding author: Department of Animal Pathology, Faculty of Veterinary Sciences, University of Santiago de Compostela, 27002, Lugo, Spain. Tel: +34 982252350 Ext. 22129. Fax: +34 982252195. E-mail: pablo.diaz@usc.es
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Summary

Faecal specimens from diarrhoeic pre-weaned calves (n=61) and lambs (n=127) collected over a 1-year period (2008–2009) at 27 cattle and 28 sheep farms in Galicia (NW Spain) were examined for the presence of Cryptosporidium oocysts and positive specimens were selected for molecular examination. Overall, 30 calves (49·2%) and 39 lambs (30·7%) tested positive for Cryptosporidium by microscopy. PCR products of the SSU rRNA locus were obtained for 27 Cryptosporidium positive calf isolates and 23 lamb isolates. Restriction analyses generated profiles of C. parvum in all isolates except for 9 lamb specimens from 5 farms that yielded banding patterns and sequences indicative of the Cryptosporidium cervine genotype. Sequence analyses of the glycoprotein (GP60) gene revealed that all but 1 C. parvum isolate from calves belonged to the subtype IIaA15G2R1 and 1 isolate was identified as the novel subtype IIaA13G1R1. Two different subtypes were identified in sheep flocks including IIaA16G3R1, which was seen in 7 lamb isolates from a single farm and subtype IIaA15G2R1, identified in 3 isolates from 2 farms. These findings suggest a limited genetic diversity within C. parvum in ruminant livestock from this geographical area, although both calves and lambs should be considered as a reservoir for zoonotic subtypes.

Keywords

CryptosporidiumcalveslambsPCR-RFLPGP60 subtypesSpain
Type
Research Article
Information
Parasitology , Volume 137 , Issue 8 , July 2010 , pp. 1187 - 1193
Copyright
Copyright © Cambridge University Press 2010

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