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Development of a single nucleotide polymorphism-based DNA marker for fall armyworm (Lepidoptera: Noctuidae) biotyping: a case study from the fall armyworm outbreak in Sri Lanka

Published online by Cambridge University Press:  27 August 2020

Dhanesha Nanayakkara Open the ORCID record for Dhanesha Nanayakkara [Opens in a new window] ,
Dimanthi Jayatilake Open the ORCID record for Dimanthi Jayatilake [Opens in a new window]  and
Suranga Kodithuwakku Open the ORCID record for Suranga Kodithuwakku [Opens in a new window]
Dhanesha Nanayakkara
Affiliation:
Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka
Dimanthi Jayatilake*
Affiliation:
Department of Agricultural Biology, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka
Suranga Kodithuwakku*
Affiliation:
Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400, Sri Lanka
*
*Corresponding authors. Emails: djayatilake@yahoo.com and surangap@pdn.ac.lk
*Corresponding authors. Emails: djayatilake@yahoo.com and surangap@pdn.ac.lk
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Abstract

Crop damage caused by fall armyworm (FAW), Spodoptera frugipera J.E. Smith (1797) (Lepidoptera: Noctuidae), has generated concern among agriculturists globally. In 2019, FAW was first reported in Sri Lanka, where it caused significant losses to corn crops. However, given that the two FAW biotypes – “rice strain” (R-FAW) and “corn strain” (C-FAW) – are morphologically identical, the biotype(s) present in Sri Lanka were unknown. The current study used the mitochondrial cytochrome c oxidase subunit I gene (mt-CO1) of FAW to biotype nine FAW samples collected in Sri Lanka. The resulting molecular phylogeny revealed that both R-FAW and C-FAW biotypes were present among the samples. In addition, we used the temperature-switch polymerase chain reaction (PCR) technique to develop a gel-based molecular marker. Two fragments were successfully amplified by the newly developed marker ABUOP0002, with fragment sizes of 341 bp from R-FAW and 204 bp from C-FAW. This demonstrates that ABUOP0002 can serve as a diagnostic gel-based molecular marker to identify the R-FAW and C-FAW biotypes from samples already identified to species level as S. frugiperda through taxonomical keys and provides a possible alternative to more expensive sequencing-based assays.

Type
Research Papers
Information
The Canadian Entomologist , Volume 152 , Issue 6 , December 2020 , pp. 762 - 771
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Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of the Entomological Society of Canada

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Footnotes

Subject editor: Amanda Roe

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