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Use of mouse oocytes to evaluate the ability of human sperm to activate oocytes after failure of activation by intracytoplasmic sperm injection

Published online by Cambridge University Press:  09 August 2004

Yasuyuki Araki ,
Midori Yoshizawa ,
Hiroyuki Abe ,
Yoshihiko Murase  and
Yasuhisa Araki
Yasuyuki Araki
Affiliation:
Science of Plant and Animal Production, United Graduated School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchuu-city, Tokyo, Japan 183-8509. Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan. The Institute for Advanced Reproductive Medical Technology, Gunma, Japan.
Midori Yoshizawa
Affiliation:
Science of Plant and Animal Production, United Graduated School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchuu-city, Tokyo, Japan 183-8509. Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Tochigi, Japan.
Hiroyuki Abe
Affiliation:
Research Institute for the Functional Peptides, Yamagata, Japan.
Yoshihiko Murase
Affiliation:
Kanayama Ladies Clinic, Nagoya, Japan.
Yasuhisa Araki
Affiliation:
The Institute for Advanced Reproductive Medical Technology, Gunma, Japan.
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Abstract

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The objective of the present study was to investigate the nuclei of human sperms that failed to fertilize human oocytes after intracytoplasmic sperm injection (ICSI). The sperms were injected into mouse oocytes by a piezo-micromanipulator, and some of these oocytes were artificially activated with strontium chloride (SrCl2) after ICSI. The oocytes were fixed, stained, and subjected to chromosomal analysis. The survival rate of mouse oocytes injected with infertile human sperms was 92.0% (46/50), while that of the control mouse oocytes injected with fertile human sperms was 73.6% (81/110). The rate of two pronuclei (2PN) formation was 0 (0/46) by the infertile sperms and 81.5% (66/81) by the fertile ones, a significant difference (p<0.01). Sperm chromosomes in non-activated oocytes were present as premature chromosome condensation (PCC). Artificial activation after ICSI increased the 2PN formation rate in the infertile group to 90.3% (28/31). The results of the present study suggest that infertile sperms have a low potential to spontaneously activate oocytes and to form pronuclei. Thus, artificial activation after ICSI may rescue oocytes fertilized with infertile human sperms that do not produce 2PN. The present study proved the usefulness of mouse oocytes as specimens in evaluating the oocyte-activating capacity of objective human sperms prior to ICSI treatment.

Keywords

ICSIInfertile spermMouse oocyteOocyte activationPCCSrCl2
Type
Research Article
Information
Zygote , Volume 12 , Issue 2 , May 2004 , pp. 111 - 116
Copyright
2004 Cambridge University Press