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Transplantation of male pronucleus derived from in vitro fertilization of enucleated oocyte into parthenogenetically activated oocyte results in live offspring in mouse

Published online by Cambridge University Press:  19 May 2005

Feng-Yun Kong ,
Gang Zhang ,
Zhi-Sheng Zhong ,
Yun-Long Li ,
Qing-Yuan Sun  and
Da-Yuan Chen
Feng-Yun Kong
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing, 100080, China. Key Laboratory of Animal Resistance, School of Life Science, Shandong Normal University, Jinan, 250014, China.
Gang Zhang
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing, 100080, China. Key Laboratory of Animal Resistance, School of Life Science, Shandong Normal University, Jinan, 250014, China.
Zhi-Sheng Zhong
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing, 100080, China.
Yun-Long Li
Affiliation:
Key Laboratory of Animal Resistance, School of Life Science, Shandong Normal University, Jinan, 250014, China.
Qing-Yuan Sun
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing, 100080, China.
Da-Yuan Chen
Affiliation:
State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing, 100080, China.
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Abstract

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In this study, inter-strain reconstructed embryos were produced by combining the female pronucleus of Kunming mouse (white) with male pronucleus of C57BL/6 strain (black). Metaphase II (MII) oocytes of Kunming mouse were enucleated and the zona pellucida was removed. Then, the enucleated oocytes were inseminated by capacitated sperm of C57BL/6 mouse in vitro. At the same time, MII oocytes of Kunming mouse were artificially activated using strontium chloride solution, which did not contain cytochalasin B. Finally, we removed the male pronucleus derived from C57BL/6 sperm and injected it into a parthenogenetically activated one-pronucleus oocyte by micromanipulation. The reconstructed 2-cell embryos were transplanted into the oviducts of 22 foster mother mice, each receiving about 20 embryos. In the end, seven healthy and live pups were born from one recipient.

Keywords

FertilizationOvumReproductive biotechnology
Type
Research Article
Information
Zygote , Volume 13 , Issue 1 , February 2005 , pp. 35 - 38
Copyright
2005 Cambridge University Press