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LacZ transgene expression as a cell marker to analyse rescue from the 2-cell block in mouse aggregation chimeras

Published online by Cambridge University Press:  01 August 1998

Irina E. Neganova ,
Martin Augustin ,
Galina G. Sekirina  and
Harald Jockusch
Irina E. Neganova
Affiliation:
Laboratory of Cell Morphology, Institute of Cytology, Russian Academy of Sciences, St Petersburg, Russia Visiting guest of SFB 223, Bielefeld, Germany
Martin Augustin
Affiliation:
Developmental Biology Unit, University of Bielefeld, D-33501 Bielefeld, Germany Present address: Fraunhofer-Institute, Department of Cell Biology, D-30625 Hannover, Germany.
Harald Jockusch
Affiliation:
Developmental Biology Unit, University of Bielefeld, D-33501 Bielefeld, Germany
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Abstract

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Embryos from certain mouse strains are arrested at the 2-cell stage in cell culture (‘2-cell block’), whereas those from other strains develop to the blastocyst stage under the same conditions. It was previously shown that blocking embryos can be rescued in culture by aggregation with an excess of 2-cell stages of a non-blocking strain such as CBA × C57BL/6 F2. Here we have employed a LacZ transgene in a blocking strain (NMRI) to follow the fate of rescued blastomeres up to the blastocyst stage. We found that rescued blastomeres can participate in both inner cell mass and trophoblast formation, thus completely overcoming the 2-cell block.

Keywords

Aggregation chimeras2-cell blockCell labellingLacZ transgene expressionPreimplantation development
Type
Research Article
Information
Zygote , Volume 6 , Issue 3 , August 1998 , pp. 223 - 226
Copyright
© 1998 Cambridge University Press