Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Isabelle Oger; Christelle Da Cruz; Gilles Panteix; Yves Menezo

doi:10.1017/S0967199403002442

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

In our work, we have used 8-hydroxy-deoxyguanosine (8-OH-dG), one of the major oxidative products of sperm DNA, in a population of patients consulting for infertility. We found an inverse relationship between sperm concentration and the log of the ratio of 8-OH-dG to dG (P<0.01). On the same patients' sperm samples, the sperm chromatin structure assay (SCSA) was performed. An inverse relationship was observed between the DNA fragmentation index and sperm concentration (P<0.001). There was also a positive relationship between SCSA and log 8-OH-dG/dG. This indicates that DNA fragmentation measured by the SCSA originates in part from oxidative products. In a few patients, antioxidant treatment decreased the DNA fragmentation index below the threshold of 30% that is crucial for subfertility.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Sergerie, M. Bleau, G. Teulé, R. Daudin, M. and Bujan, L. 2005. Intégrité de l'ADN des spermatozoïdes comme élément diagnostique et pronostique de la fertilité masculine. Gynécologie Obstétrique & Fertilité, Vol. 33, Issue. 3, p. 89.

Ménézo, Yves and Guérin, Pierre 2005. Protection des gamètes et de l’embryon contre le stress oxydatif en procréation médicale assistée. Bulletin de l'Académie Nationale de Médecine, Vol. 189, Issue. 4, p. 715.

Menezo, D’après la communication de Y. 2006. L’ovocyte réparateur de l’ADN du spermatozoïde. Journal de Gynécologie Obstétrique et Biologie de la Reproduction, Vol. 35, Issue. 5, p. 19.

Loutradi, Kalliopi E. Tarlatzis, Basil C. Goulis, Dimitrios G. Zepiridis, Leonidas Pagou, Thoula Chatziioannou, Elisabeth Grimbizis, Grigoris F. Papadimas, Ioannis and Bontis, Ioannis 2006. The effects of sperm quality on embryo development after intracytoplasmic sperm injection. Journal of Assisted Reproduction and Genetics, Vol. 23, Issue. 2, p. 69.

2006. The clinical utility of sperm DNA integrity testing. Fertility and Sterility, Vol. 86, Issue. 5, p. S35.

Zini, Armand and Libman, Jamie 2006. Sperm DNA damage: importance in the era of assisted reproduction. Current Opinion in Urology, Vol. 16, Issue. 6, p. 428.

Silva, P.F.N. and Gadella, B.M. 2006. Detection of damage in mammalian sperm cells. Theriogenology, Vol. 65, Issue. 5, p. 958.

Aitken, R.J. Roman, S.D. Baker, M.A. and De Iuliis, G. 2007. Male-mediated Developmental Toxicity. p. 197.

Zhang, Xiaoyang San Gabriel, Maria Libman, Jamie Phillips, Simon Courchesne, Annick and Zini, Armand 2007. Localization of single-stranded DNA in human sperm nuclei. Fertility and Sterility, Vol. 88, Issue. 5, p. 1334.

El-Mouatassim, Said Bilotto, Stefania Russo, Gian Luigi Tosti, Elisabetta and Menezo, Yves 2007. APEX/Ref-1 (apurinic/apyrimidic endonuclease DNA-repair gene) expression in human and ascidian (Ciona intestinalis) gametes and embryos *. MHR: Basic science of reproductive medicine, Vol. 13, Issue. 8, p. 549.

Cocuzza, Marcello Sikka, Suresh C. Athayde, Kelly S. and Agarwal, Ashok 2007. Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis. International braz j urol, Vol. 33, Issue. 5, p. 603.

Chakroun Feki, Nozha Zribi, Nassira Eleuch, Henda Gdoura, Radouane Sellami, Afifa Bahloul, Ali Hammami, Adnene Gargouri, Jalel Rebai, Tarek and Keskes Ammar, Leila 2008. Intérêt de l’étude de l’oxydation de l’ADN des spermatozoïdes par marquage de la 8-oxo-guanine en cytométrie en flux chez l’homme infertile. Andrologie, Vol. 18, Issue. 3, p. 197.

Badouard, C. Ménézo, Y. Panteix, G. Ravanat, J.L. Douki, T. Cadet, J. and Favier, A. 2008. Determination of new types of DNA lesions in human sperm. Zygote, Vol. 16, Issue. 1, p. 9.

Tremellen, Kelton 2008. Oxidative stress and male infertility—a clinical perspective. Human Reproduction Update, Vol. 14, Issue. 3, p. 243.

Meseguer, Marcos Martínez-Conejero, J.A. O'Connor, J. Enrique Pellicer, Antonio Remohí, José and Garrido, Nicolás 2008. The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility prognostic factor. Fertility and Sterility, Vol. 89, Issue. 5, p. 1191.

Agbaje, IM McVicar, CM Schock, BC McClure, N Atkinson, AB Rogers, D and Lewise, SEM 2008. Increased concentrations of the oxidative DNA adduct 7,8-dihydro-8-oxo-2-deoxyguanosine in the germ-line of men with type 1 diabetes. Reproductive BioMedicine Online, Vol. 16, Issue. 3, p. 401.

2008. The clinical utility of sperm DNA integrity testing. Fertility and Sterility, Vol. 90, Issue. 5, p. S178.

Aguilar, Cristobal Garrido, Nicolas Viloria, Thamara Luis Fernandez, Jose and Meseguer, Marcos 2008. Biochemical markers of male infertility: the key role of DNA damage. Expert Review of Obstetrics & Gynecology, Vol. 3, Issue. 4, p. 565.

De Iuliis, Geoffry N. Newey, Rhiannon J. King, Bruce V. Aitken, R. John and Zhang, Baohong 2009. Mobile Phone Radiation Induces Reactive Oxygen Species Production and DNA Damage in Human Spermatozoa In Vitro. PLoS ONE, Vol. 4, Issue. 7, p. e6446.

Aguilar, Cristóbal Meseguer, Marcos García-Herrero, Sandra Gil-Salom, Manuel O'Connor, José E. and Garrido, Nicolás 2010. Relevance of testicular sperm DNA oxidation for the outcome of ovum donation cycles. Fertility and Sterility, Vol. 94, Issue. 3, p. 979.

Download full list

Article contents

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Abstract

Keywords

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Article contents

Evaluating human sperm DNA integrity: relationship between 8-hydroxydeoxyguanosine quantification and the sperm chromatin structure assay

Abstract

Keywords

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests