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Activity of exoglycosidases in ejaculated spermatozoa of boar and bull

Published online by Cambridge University Press:  09 August 2004

Masayuki Hayashi ,
Naoto Yonezawa ,
Toshiyuki Katsumata ,
Keiichi Ikeda ,
Fabiana Lica Imai ,
Kazuhiro Kikuchi ,
Seizo Hamano  and
Minoru Nakano
Masayuki Hayashi
Affiliation:
Graduate School of Science and Technology, Chiba University, Chiba, 263-8522, Japan.
Naoto Yonezawa
Affiliation:
Graduate School of Science and Technology, Chiba University, Chiba, 263-8522, Japan. Department of Chemistry, Faculty of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.
Toshiyuki Katsumata
Affiliation:
College of Liberal Arts and Science, Tokyo Medical and Dental University, Ichikawa, 272-0827, Japan.
Keiichi Ikeda
Affiliation:
Graduate School of Science and Technology, Chiba University, Chiba, 263-8522, Japan.
Fabiana Lica Imai
Affiliation:
Graduate School of Science and Technology, Chiba University, Chiba, 263-8522, Japan.
Kazuhiro Kikuchi
Affiliation:
Department of Genetic Resources II, National Institute of Agrobiological Resources, Tsukuba, 305-8602, Japan.
Seizo Hamano
Affiliation:
Animal Bio-Technology Center, Livestock Improvement Association, Tokyo, 140-0002, Japan.
Minoru Nakano
Affiliation:
Graduate School of Science and Technology, Chiba University, Chiba, 263-8522, Japan. Department of Chemistry, Faculty of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan.
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Abstract

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The activity of exoglycosidases in extracts from freshly ejaculated boar and bull spermatozoa with 0.2% Brij-35/2% acetic acid was measured. The results show that β-N-acetylhexosaminidase, β-galactosidase and α-mannosidase are the major glycosidases; much higher levels of activity were found in boar spermatozoa than in bull spermatozoa. When compared on a per spermatozoon basis, the ratios of the activities of β-N-acetylhexosaminidase, β-galactosidase and α-mannosidase in boar spermatozoon relative to those in bull spermatozoon were approximately 13 000:1, 1700:1 and 400:1, respectively. Liberation of these glycosidases from bull spermatozoa by treatment with phosphatidylinositol-specific phospholipase C (PI-PLC) was low, in contrast to liberation of α-mannosidase from boar spermatozoa previously found by the same means. The possibility that the exoglycosidases present in large amounts in boar spermatozoa play a role in the process of binding to the zona pellucida glycoprotein of the egg is discussed.

Keywords

Acrosome reactionGPI anchorSperm glycosidaseSperm–egg recognitionZona pellucida
Type
Short Communication
Information
Zygote , Volume 12 , Issue 2 , May 2004 , pp. 105 - 109
Copyright
2004 Cambridge University Press