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Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation

Published online by Cambridge University Press:  09 April 2002

K. Sugiura ,
K. Naito ,
N. Iwamori ,
H. Kagii ,
S. Goto ,
S. Ohashi ,
H. Naruoka ,
E. Yada ,
K. Yamanouchi  and
H. Tojo
K. Sugiura
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
K. Naito
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
N. Iwamori
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
H. Kagii
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
S. Goto
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
S. Ohashi
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
H. Naruoka
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
E. Yada
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
K. Yamanouchi
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
H. Tojo
Affiliation:
Laboratory of Applied Genetics, Department of Animal Resource Sciences, Graduate School of Agricultural Sciences, University of Tokyo, Tokyo 113-8657, Japan
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Abstract

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The normal kinetics of ribosomal S6 kinase (RSK) during the meiotic maturation of porcine oocytes were examined. The phosphorylation states of RSK and extracellular signal-regulated kinase (ERK), major mitogen-activated protein (MAP) kinases in maturating porcine oocytes, were detected by Western blotting analysis. The S6 protein kinase activity was assayed using a specific substrate peptide which contained the major phosphorylation sites of S6 kinase. Full phosphorylation of RSK was correlated with ERK phosphorylation and was observed before germinal vesicle breakdown. S6 kinase activity was low in both freshly isolated and 20 h cultured oocytes. S6 kinase activity was significantly elevated in matured oocytes to a level about 6 times higher than that in freshly isolated oocytes. Furthermore, full phosphorylation of RSK was inhibited when oocytes were treated with U0126, a specific MAP kinase kinase inhibitor, in dose-dependent manner, indicating that RSK is one of the substrates of MAP kinase. These results suggest that the activation of RSK is involved in the regulation of meiotic maturation of porcine oocytes.

Keywords

Oocyte maturationPorcine oocyteRSKS6 kinase
Type
Research Article
Information
Zygote , Volume 10 , Issue 1 , February 2002 , pp. 31 - 36
Copyright
2002 Cambridge University Press