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Nontarget-Site Resistance to ALS Inhibitors in Waterhemp (Amaranthus tuberculatus)

Published online by Cambridge University Press:  20 January 2017

Jiaqi Guo ,
Chance W. Riggins ,
Nicholas E. Hausman ,
Aaron G. Hager ,
Dean E. Riechers ,
Adam S. Davis  and
Patrick J. Tranel
Jiaqi Guo
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Chance W. Riggins
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Nicholas E. Hausman
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Aaron G. Hager
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Dean E. Riechers
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Adam S. Davis
Affiliation:
U.S. Department of Agriculture–Agriculture Research Service, Urbana, IL 61801
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@illinois.edu.
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Abstract

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A waterhemp population (MCR) previously characterized as resistant to 4-hydroxyphenylpyruvate dioxygenase and photosystem II inhibitors demonstrated both moderate and high levels of resistance to acetolactate synthase (ALS) inhibitors. Plants from the MCR population exhibiting high resistance to ALS inhibitors contained the commonly found Trp574Leu ALS amino acid substitution, whereas plants with only moderate resistance did not have this substitution. A subpopulation (JG11) was derived from the MCR population in which the moderate-resistance trait was isolated from the Trp574Leu mutation. Results from DNA sequencing and ALS enzyme assays demonstrated that resistance to ALS inhibitors in the JG11 population was not due to an altered site of action. This nontarget-site ALS-inhibitor resistance was characterized with whole-plant dose–response experiments using herbicides from each of the five commercialized families of ALS-inhibiting herbicides. Resistance ratios ranging from 3 to 90 were obtained from the seven herbicides evaluated. Nontarget-site resistance to ALS has been rarely documented in eudicot weeds, and adds to the growing list of resistance traits evolved in waterhemp.

Keywords

Waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif AMATUHerbicide metabolismherbicide resistancemalathionresistance mechanismscommon waterhemptall waterhemp
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 63 , Issue 2 , June 2015 , pp. 399 - 407
Copyright
Copyright © Weed Science Society of America 

References

Literature Cited

Bell, MS, Hager, AG, Tranel, PJ (2013) Multiple resistance to herbicides from four site-of-action groups in waterhemp (Amaranthus tuberculatus). Weed Sci. 61:460–468 CrossRefGoogle Scholar
Costea, M, Weaver, SE, Tardif, FJ (2005) The biology of invasive alien plants in Canada. 3. Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea & Tardif. Can J Plant Sci. 85:507–522 Google Scholar
Délye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Manag Sci. 69:176–187 CrossRefGoogle Scholar
Délye, C, Pernin, F, Scarabel, L (2011) Evolution and diversity of the mechanisms endowing resistance to herbicides inhibiting acetolactate-synthase (ALS) in corn poppy (Papaver rhoeas L.). Plant Sci. 180:333–342 CrossRefGoogle ScholarPubMed
Diebold, RS, McNaughton, KE, Lee, EA, Tardif, FJ (2003) Multiple resistance to imazethapyr and atrazine in Powell amaranth (Amaranthus powellii). Weed Sci. 51:312–318 CrossRefGoogle Scholar
Duggleby, RG, Pang, SS (2000) Acetohydroxyacid synthase. J Biochem Mol Biol. 33:1–36 Google Scholar
Foes, MJ, Liu, L, Tranel, PJ, Wax, LM, Stoller, EW (1998) A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci. 46:514–520 CrossRefGoogle Scholar
Gaines, TA, Lorentz, L, Figge, A, Herrmann, J, Maiwald, F, Ott, MC, Han, H, Busi, R, Yu, Q, Powles, SB, Beffa, R (2014) RNA-Seq transcriptome analysis to identify genes involved in metabolism-based diclofop resistance in Lolium rigidum . Plant J. 78:865–876 Google Scholar
Hausman, NE, Singh, S, Tranel, PJ, Riechers, DE, Kaundun, SS, Polge, ND, Thomas, DA, Hager, AG (2011) Resistance to HPPD-inhibiting herbicides in a population of waterhemp (Amaranthus tuberculatus) from Illinois, United States. Pest Manag Sci. 67:258–261 Google Scholar
Hausman, NE, Tranel, PJ, Riechers, DE, Maxwell, DJ, Gonzini, LC, Hager, AG (2013) Responses of an HPPD inhibitor-resistant waterhemp (Amaranthus tuberculatus) population to soil-residual herbicides. Weed Technol. 27:704–711 Google Scholar
Heap, I (2014) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed June 11, 2014Google Scholar
Iwakami, S, Endo, M, Saika, H, Okuno, J, Nakamura, N, Yokoyama, M, Watanabe, H, Toki, S, Uchino, A, Inamura, T (2014) Cytochrome P450 CYP81A12 and CYP81A21 are associated with resistance to two acetolactate synthase inhibitors in Echinochloa phyllopogon . Plant Physiol. 165:618–629 CrossRefGoogle ScholarPubMed
Iwakami, S, Uchino, A, Kataoka, Y, Shibaike, H, Watanabe, H, Inamura, T (2013) Cytochrome P450 genes induced by bispyribac-sodium treatment in a multiple-herbicide-resistant biotype of Echinochloa phyllopogon . Pest Manag Sci. 70:549–558 CrossRefGoogle Scholar
Jeffers, GM, O'Donovan, JT, Hall, LM (1996) Wild mustard (Brassica kaber) resistance to ethametsulfuron-methyl but not to other herbicides. Weed Technol. 10:847–850 Google Scholar
Knezevic, SZ, Streibig, JC, Ritz, C (2007) Utilizing R software package for dose–response studies: the concept and data analysis. Weed Technol. 21:840–848 Google Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoe, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol. 163:363–377 CrossRefGoogle Scholar
McNaughton, KE, Letarte, J, Lee, EA, Tardif, FJ (2005) Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci. 53:17–22 Google Scholar
Patzoldt, WL, Tranel, PJ (2007) Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci. 55:421–428 Google Scholar
Patzoldt, WL, Tranel, PJ, Hager, AG (2005) A waterhemp (Amaranthus tuberculatus) biotype with multiple resistance across three herbicide sites of action. Weed Sci. 53:30–36 CrossRefGoogle Scholar
R Core Team (2013) A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. http://www.R-project.org/. Accessed April 15, 2013.Google Scholar
Riggins, CW, Peng, Y, Stewart, CN Jr., Tranel, PJ (2010) Characterization of de novo transcriptome for waterhemp (Amaranthus tuberculatus) using GS-FLX 454 pyrosequencing and its application for studies of herbicide target-site genes. Pest Manag Sci. 66:1042–1052 CrossRefGoogle ScholarPubMed
Schmitzer, PR, Eilers, RJ, Cséke, C (1993) Lack of cross-resistance of imazaquin-resistant Xanthium strumarium acetolactate synthase to flumetsulam and chlorimuron. Plant Physiol. 103:281–283 Google Scholar
Steckel, LE (2007) The dioecious Amaranthus spp.: here to stay. Weed Technol. 21:567–570 CrossRefGoogle Scholar
Tranel, PJ, Jiang, W, Patzoldt, WL, Wright, TR (2004) Intraspecific variability of the acetolactate synthase gene. Weed Sci. 52:236–241 Google Scholar
Tranel, PJ, Riggins, CW, Bell, MS, Hager, AG (2011) Herbicide resistances in Amaranthus tuberculatus: a call for new options. J Agric Food Chem. 59:5808–5812 Google Scholar
Tranel, PJ, Wright, TR (2002) Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700–712 Google Scholar
Tranel, PJ, Wright, TR, Heap, IM (2014) Mutations in Herbicide-Resistant Weeds to ALS Inhibitors. http://weedscience.org/mutations/mutationdisplayall.aspx. Accessed: June 11, 2014Google Scholar
Veldhuis, LJ, Hall, LM, O'Donovan, JT, Dyer, W, Hall, JC (2000) Metabolism-based resistance of a wild mustard (Sinapis arvensis L.) biotype to ethametsulfuron-methyl. J Agric Food Chem. 48:2986–2990 Google Scholar
Yu, Q, Powles, SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci. 70:1340–1350 Google Scholar