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Quantifying protozoal duodenal outflow in steers by real-time PCR

Published online by Cambridge University Press:  23 November 2017

D.R. Yáñez Ruiz ,
N.D. Scollan ,
R.J. Merry  and
C.J. Newbold
D.R. Yáñez Ruiz*
Affiliation:
institute of Rural Sciences, University of Wales, Aberystwyth SY23 3AL, U.K.
N.D. Scollan
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, U.K.
R.J. Merry
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB, U.K.
C.J. Newbold
Affiliation:
institute of Rural Sciences, University of Wales, Aberystwyth SY23 3AL, U.K.
*
Email: dyy@aber.ac.uk
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Extract

Microbial protein contributes about two-thirds of the amino acids absorbed by ruminants. Information on the proportion of bacterial and protozoal N passing to the duodenum would enhance our understanding of the effect of diet not only on microbial protein synthesis but also on the contribution of bacteria and protozoa to duodenal flow of others metabolites of interest. However, differentiation of the duodenal fractions has proven to be difficult because routine procedures cannot separate microbial protein at the duodenum into bacteria and protozoa (Punia et al., 1992). Molecular techniques that use group-specific rRNA-targeted probes may help overcome these problems. The objective of this experiment was to quantify the flow of protozoal N at the duodenum in steers, fed two silage diets differing in water soluble carbohydrates (WSC) content, by real-time PCR using protozoa specific primers.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2005 , 2005 , pp. 70
Copyright
Copyright © The British Society of Animal Science 2005

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