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Maturation of porcine oocytes for 33 or 44 hours in vitro in the presence or absence of serum

Published online by Cambridge University Press:  05 November 2021

K. Rust ,
M.E. Staines ,
GJ. McCallum ,
N.S. Prathalingam ,
S.A. Edwards ,
P.R. English  and
T.G. McEvoy
K. Rust
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB9 1UD, United Kingdom
M.E. Staines
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom
GJ. McCallum
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom
N.S. Prathalingam
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom
S.A. Edwards
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom
P.R. English
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street, Aberdeen AB9 1UD, United Kingdom
T.G. McEvoy
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB219YA, United Kingdom
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Extract

Porcine embryo production in vitrois providing the impetus for the development of cryopreservation strategies aimed at welfare-friendly domestic and international marketing and movement of stock in a manner that minimises risks of disease transmission. In the context of disease risks, defined media, which avoid the use of serum and other biohazardous products, are likely to become essential in the production of embryos for international markets. In preparing for this situation, the present comparative study investigated in vitronuclear maturation of porcine oocytes in the presence of either foetal calf serum (FCS) or polyvinyl alcohol (PVA). In addition, the effect of restricting the maturation time to 33 rather than 44 hours was examined.

Type
Programme
Information
Proceedings of the British Society of Animal Science , Volume 1998 , 1998 , pp. 180
Copyright
Copyright © The British Society of Animal Science 1998

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References

Staines, M.E., Dolman, D.F., Watt, R.G., Higgins, L.C., McCallum, G.J. and McEvoy, T.G. (1997). Bovine oocyte maturation in a serum-free system does not compromise blastocyst yields in vitro. Journal of Reproduction and Fertility. Abstract Series 20. paper no. 5.Google Scholar