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Mammary specific function of a bovine mammary epithelial cell (ABERMEC) clone cultured on collagen I coated inserts in the presence and absence of foetal bovine serum

Published online by Cambridge University Press:  23 November 2017

H.R. M ,
M.T. Rose ,
H. Aso ,
W. Haresign  and
B. Davies
H.R. M*
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL, U.K.
M.T. Rose
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL, U.K.
H. Aso
Affiliation:
Graduate School of Agricultural Science, Tohoku University, Aoba-Ku, Sendai-shi, Japan
W. Haresign
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL, U.K.
B. Davies
Affiliation:
Institute of Rural Sciences, Llanbadarn Campus, Llanbadarn Fawr, Ceredigion, SY23 3AL, U.K.
*
Email: hrm98@aber.ac.uk
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Extract

The aim was to establish a representative model of the bovine mammary gland in order to underpin applied research in mammary gland development and lactation. Cell culture insert methodology is currently being utilized in place of a three dimensional culture system, the shortcomings of which have been discussed elsewhere (McConochie et al., 2004). Cell culture insert methodology offers a promising alternative, with the potential to recreate in vitro a polarised epithelial layer. Previously it has been shown that on collagen I coated inserts, ABERMEC are able to synthesise and secrete mammary specific proteins in the apparent absence of the key mediators laminin and prolactin. It was hypothesised that undefined factors in serum were a possible cause for this phenomenon.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2005 , 2005 , pp. 39
Copyright
Copyright © The British Society of Animal Science 2005

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References

McConochie, H.R., Rose, M.T., Haresign, W. and Davies, B. 2004. Mammary specific function of a bovine mammary epithelial cell clone cultured on collagen I coated inserts. Jour. of Animal and Feed Sciences 13: 523–526 Supplement 1.Google Scholar
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