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Characterization of transcribed ovine lymphocyte antigen (OLA) class I genes by single strand conformational polymorphism (SSCP) and sequence analysis

Published online by Cambridge University Press:  20 November 2017

D. Miltiadou ,
K. T. Ballingall ,
S. A. Ellis  and
D. J. McKeever
D. Miltiadou*
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, U.K.
K. T. Ballingall
Affiliation:
Division of Veterinary Clinical Studies, Easter Bush, Roslin, Midlothian, EH25 9RG, U.K.
S. A. Ellis
Affiliation:
Institute for Animal Health, Compton, RG20 7NN, U.K.
D. J. McKeever*
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, U.K. Division of Veterinary Clinical Studies, Easter Bush, Roslin, Midlothian, EH25 9RG, U.K.
*
E-mail: Despoina.Miltiadou@mri.sari.ac.uk
E-mail: Declan.McKeever@mri.sari.ac.uk
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Extract

The Major Histocompatibility Complex (MHC) class I genes encode polymorphic cell surface glycoproteins that initiate cytotoxic immune responses by presenting processed antigenic peptides to CD8+ T cells. Due to their functional role and polymorphic nature, MHC class I genes have been used in disease association and vaccine development studies in many species. A prerequisite for such studies is the availability of animals of defined MHC haplotypes. However, the ovine MHC (OLA) class I genes have been studied largely by serology and very limited information is available at the DNA level (Dietert R., 1996). In this study, we aimed to identify the number of transcribed OLA class I genes in two Blackface rams and to obtain sequence data for exons 2 and 3. These data will be used to establish an allele specific PCR based typing system to genotype a cohort of progeny for each ram. This will enable allocation of alleles to individual haplotypes and inform a breeding strategy for the generation of MHC defined animals for functional and disease association studies.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 199
Copyright
Copyright © The British Society of Animal Science 2004

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References

Ainsworth, P. J., Surh, L. C. and Coulter-Mackie, M. B. 1991. Diagnostic single strand conformational polymorphism, (SSCP): a simplified non-radioisotopic method as applied to a Tay-Sachs B1 variant. Nucleic Acids Research 19 (2):405–406.Google Scholar
Dietert, R. R. 1996. The ovine major histocompatibility complex . In The Major Histocompatibility Complex Region of Domestic Animals. Edited by Schook, LB. and Lamont, SJ. CRC Press. Chapter 4: 65–98.Google Scholar
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