Hostname: page-component-6bf8c574d5-xtvcr Total loading time: 0 Render date: 2025-02-22T10:16:27.392Z Has data issue: false hasContentIssue false
  • English
  • Français

Changes in total and individual proteins during drying and ruminal fermentation of alfalfa

Published online by Cambridge University Press:  23 November 2017

A. A. Sadeghi ,
P. Shawrang ,
M. Moradi  and
A. Nikkhah
A. A. Sadeghi*
Affiliation:
Department of Animal Science, Science & Research Campus, Islamic Azad University, Tehran, Iran
P. Shawrang*
Affiliation:
Dep. of Animal Science, Tehran University, Karaj, Iran
M. Moradi
Affiliation:
Dep. of Animal Science, Tehran University, Karaj, Iran
A. Nikkhah
Affiliation:
Dep. of Animal Science, Tehran University, Karaj, Iran
*
Email: maryamsa1381@yahoo.com
Email: pshawrang@yahoo.com
Get access

Extract

Proteolysis within plant cells occurs during wilting and drying. Changes in plant proteins during those periods usually are monitored by measurement of total crude protein and non protein nitrogen. Alternatively, changes in concentrations of individual proteins can be measured. Plants are composed of an array of different proteins. Electrophoresis can be used to separate these proteins and has been used to study effects of wilting and ensiling on proteins of some forages (Grum et al., 1991). Electrophoresis also has been used in the study of ruminal hydrolysis of oilseed meals proteins (Sadeghi et al., 2004). Most of the experiments designed to use electrophoresis to study protein metabolism in forages and ruminants have been qualitative. The main objective of this study was to determine whether sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and densitometry could be used to monitor quantitatively the changes in alfalfa protein composition during wilting, drying and ruminal exposure.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2005 , 2005 , pp. 198
Copyright
Copyright © The British Society of Animal Science 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

AFRC, 1992. Nutrient requirements of ruminant animals: protein. Report No. 9. Nutrition abstracts and reviews series B 62: 787–835.Google Scholar
Grum, D. E., Shockey, W. L., and Weiss, W. P. 1991. Electrophoretic examination of alfalfa silage proteins. Journal of Dairy Science 74: 146–154.CrossRefGoogle Scholar
Laemmli, U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227: 680.CrossRefGoogle ScholarPubMed
Sadeghi, A. A., Nikkhah, A., Shahrebabak, M. M. and Shawrang, P. 2004. Protein degradation kinetics of untreated and xylose treated soya bean meal by using SDS-PAGE. Proceedings of British Society of Animal Science. pp 233.Google Scholar