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Comparison of methods for the detemination of peptides nitrogen in grass silage

Published online by Cambridge University Press:  05 November 2021

V. L. Nsereko  and
J. A. Rooke
V. L. Nsereko
Affiliation:
Animal Biology Division, SAC, Ferguson Building, Craibstone Estate, Aberdeen AB24 SUA
J. A. Rooke
Affiliation:
Animal Biology Division, SAC, Ferguson Building, Craibstone Estate, Aberdeen AB24 SUA
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Extract

There are few reports on the occurrence and amounts of peptide-N in silage. Previous studies (Nsereko and Rooke, 1993, 1996) have quantified the amounts of peptide-N in grass silage and how peptide-N can be modified by additive treatment. However, peptide N concentrations determined using fluorescamine were observed to be considerably higher than those measured by amino acid analysis (Nsereko, 1996). It was the objective of this study to compare methods for the analysis of peptide-N.

Triplicate silages treated with no additive, formic acid (5.4 g/kg) or formic acid (2.7 g/kg) and formaldehyde (3.75 g/kg) (Nsereko and Rooke, 1993) were used in an initial study to compare three methods of peptide analysis. Aqueous extracts were prepared from the silages which were deproteinised with trichloracetic acid.

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Proceedings of the British Society of Animal Science , Volume 1998 , 1998 , pp. 152
Copyright
Copyright © The British Society of Animal Science 1998

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References

Nsereko, V.L. and Rooke, J.A. (1993) The effect of novel additives upon protein breakdown during ensilage. Proceedings of the 10th International Silage Conference, pp 116–117.Google Scholar
Nsereko, V. L. and Rooke, J.A. (1996) The effects of peptidase inhibitors on protein degradation during ensilage. Proceedings of the XIth International Silage Conference pp. 122–123.Google Scholar
Nsereko, V. L. (1996) Peptidase inhibitors as additives for silage. Ph. D. Thesis, University of Aberdeen.Google Scholar