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Use of differential display to detect changes in gene expression in the intermediate snail host Biomphalaria glabrata upon infection with Schistosoma mansoni

Published online by Cambridge University Press:  01 April 2000

A. E. LOCKYER ,
C. S. JONES ,
L. R. NOBLE  and
D. ROLLINSON
A. E. LOCKYER
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
C. S. JONES
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
L. R. NOBLE
Affiliation:
Zoology Department, Aberdeen University, Tillydrone Avenue, Aberdeen AB24 2TZ
D. ROLLINSON
Affiliation:
Biomedical Parasitology Division, Zoology Department, Natural History Museum, London SW7 5BD
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Abstract

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Changes in gene expression in Biomphalaria glabrata following infection with Schistosoma mansoni have been investigated using a modified differential display approach. RNA was extracted from ovotestis, mantle tissue and anterior nephridium of control and exposed snails at 2 time-points (4 h and 24 h) post-exposure and analysed by RNA fingerprinting. A number of transcripts were identified; some novel and some homologous to mRNAs in GenEMBL that were previously unknown in B. glabrata. Down regulation of one 241 bp mRNA expressed sequence fragment – with an open reading frame showing 48% identity to a cytochrome p450 over 80 residues – has been confirmed using semi-quantitative RT-PCR. Preliminary classification of B. glabrata cyp450 sequence shows it to fall into CLAN 2 of the cytochrome p450 superfamily. Differential display has been successful in identifying changes in gene expression in Biomphalaria glabrata upon infection with Schistosoma mansoni and promises to be a useful technique for the investigation of the interaction between host and parasite.

Keywords

Biomphalaria glabrataSchistosoma mansonidifferential displaycytochrome p450polymerase chain reaction
Type
Research Article
Information
Parasitology , Volume 120 , Issue 4 , April 2000 , pp. 399 - 407
Copyright
2000 Cambridge University Press