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Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus

Published online by Cambridge University Press:  01 January 1997

J. C. SCOTT ,
J. STEFANIAK ,
Z. S. PAWLOWSKI  and
D. P. McMANUS
J. C. SCOTT
Affiliation:
Molecular Parasitology Unit, Tropical Health Program, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane, Queensland 4029, Australia
J. STEFANIAK
Affiliation:
Clinic of Parasitic and Tropical Diseases, University of Medical Sciences, Przybyszewskiego 49, 60–355 Poznan, Poland
Z. S. PAWLOWSKI
Affiliation:
Clinic of Parasitic and Tropical Diseases, University of Medical Sciences, Przybyszewskiego 49, 60–355 Poznan, Poland
D. P. McMANUS
Affiliation:
Molecular Parasitology Unit, Tropical Health Program, Australian Centre for International and Tropical Health and Nutrition, The Queensland Institute of Medical Research, The Bancroft Centre, 300 Herston Road, Brisbane, Queensland 4029, Australia
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Abstract

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We have used nuclear (ribosomal ITS1) and mitochondrial (ND1) sequences to characterize human and pig isolates of Echinococcus granulosus collected by fine needle aspiration biopsy (FNAB) in Poland. The data indicate clearly that the Polish patients were not infected with the common sheep strain (G1 genotype) of E. granulosus, normally associated with human cystic hydatid infection. Instead, the hydatid parasite infecting the Polish patients shares very similar ND1 sequence with the previously characterized pig (G7) genotype but it also exhibits some clear differences. In particular, E. granulosus DNA from the Polish patients amplified a single ITS1 fragment in PCR and distinct ITS1–RFLP patterns were obtained after restriction digestion. The form of hydatid isolated from the Polish patients appears, therefore, to represent a distinct, previously undescribed genotype (designated G9) of E. granulosus.

Keywords

Echinococcus granulosushuman hydatid diseasecystic hydatidosisNADH dehydrogenase I genePCR-RFLP analysis
Type
Research Article
Information
Parasitology , Volume 114 , Issue 1 , January 1997 , pp. 37 - 43
Copyright
© 1997 Cambridge University Press