In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

H. A. ZAKAI; M. L. CHANCE; P. A. BATES

doi:10.1017/S0031182097002382

Abstract

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Promastigotes of Leishmania braziliensis, L. donovani, L. major and L. mexicana recently derived from tissue amastigotes were cultured in Schneider's Drosophila medium supplemented with 20% (v/v) heat-inactivated foetal calf serum and 25 μg gentamicin sulfate/ml at pH 5·5. These cultures produced more metacyclic promastigotes in their stationary-phase populations than others cultured at pH 7·0. Metacyclic promastigotes possessed a short ([les ]8 μm) and narrow ([les ]1·5 μm) cell body with a flagellum twice or more the length of the cell body. Promastigotes from acidic cultures were more resistant to complement-mediated lysis and more infective in vivo than those grown at neutral pH. These results demonstrate that induction of metacyclogenesis by acidic pH is a response conserved across a variety of species of Leishmania.

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Sacks, David L. and Melby, Peter C. 1998. Animal Models for the Analysis of Immune Responses to Leishmaniasis. Current Protocols in Immunology, Vol. 28, Issue. 1,

Mahoney, Ashley Bray and Turco, Salvatore J. 1999. Characterization of the Glucosyltransferases That Assemble the Side Chains of the Indian Leishmania donovani Lipophosphoglycan. Archives of Biochemistry and Biophysics, Vol. 372, Issue. 2, p. 367.

Hommel, Marcel 1999. Visceral leishmaniasis: biology of the parasite. Journal of Infection, Vol. 39, Issue. 2, p. 101.

Zakai, H. A. and Zimmo, S. K. 2000. Effects of itraconazole and terbinafine onLeishmania majorlesions in BALB/c mice. Annals of Tropical Medicine & Parasitology, Vol. 94, Issue. 8, p. 787.

Späth, Gerald F. and Beverley, Stephen M. 2001. A Lipophosphoglycan-Independent Method for Isolation of Infective Leishmania Metacyclic Promastigotes by Density Gradient Centrifugation. Experimental Parasitology, Vol. 99, Issue. 2, p. 97.

Kaufmann, S. H. E. Kébaı̈er, C. Louzir, H. Chenik, M. Salah, A. Ben and Dellagi, K. 2001. Heterogeneity of WildLeishmania majorIsolates in Experimental Murine Pathogenicity and Specific Immune Response. Infection and Immunity, Vol. 69, Issue. 8, p. 4906.

Ramalho-Ortigão, JM Temporal, P Oliveira, SMP de Barbosa, AF Vilela, ML Rangel, EF Brazil, RP and Traub-Cseko, YM 2001. Characterization of constitutive and putative differentially expressed mRNAs by means of expressed sequence tags, differential display reverse transcriptase-PCR and randomly amplified polymorphic DNA-PCR from the sand fly vector Lutzomyia longipalpis. Memórias do Instituto Oswaldo Cruz, Vol. 96, Issue. 1, p. 105.

Rodriguez, Noris M. Docampo, Roberto Lu, Hong-gang and Scott, David A. 2002. Overexpression of the Leishmania amazonensis Ca2+-ATPase gene lmaa1 enhances virulence. Cellular Microbiology, Vol. 4, Issue. 2, p. 117.

Jamjoom, M. B. Ashford, R. W. Bates, P. A. Kemp, S. J. and Noyes, H. A. 2002. Towards a standard battery of microsatellite markers for the analysis of theLeishmania donovanicomplex. Annals of Tropical Medicine & Parasitology, Vol. 96, Issue. 3, p. 265.

Somanna, Ashwini Mundodi, Vasanthakrishna and Gedamu, Lashitew 2002. In vitro cultivation and characterization of Leishmania chagasi amastigote-like forms. Acta Tropica, Vol. 83, Issue. 1, p. 37.

Gossage, Sharon M Rogers, Matthew E and Bates, Paul A 2003. Two separate growth phases during the development of Leishmania in sand flies: implications for understanding the life cycle. International Journal for Parasitology, Vol. 33, Issue. 10, p. 1027.

SILVA, N. CAMACHO, N. FIGARELLA, K. and PONTE-SUCRE, A. 2004. Cell differentiation and infectivity ofLeishmania mexicanaare inhibited in a strain resistant to an ABC-transporter blocker. Parasitology, Vol. 128, Issue. 6, p. 629.

Wakid, Majed H. and Bates, Paul A. 2004. Flagellar attachment of Leishmania promastigotes to plastic film in vitro. Experimental Parasitology, Vol. 106, Issue. 3-4, p. 173.

Choisy, Marc Hide, Mallorie Bañuls, Anne-Laure and Guégan, Jean-François 2004. Rocking the curve. Trends in Microbiology, Vol. 12, Issue. 12, p. 534.

Ali, Mirjalili and Bahador, Sarkari 2005. Isolation of infective promastigotes of Leishmania major from long-term culture by cocultivation with macrophage cell line. Biologicals, Vol. 33, Issue. 4, p. 257.

Soares, Rodrigo P.P. Cardoso, Tatiana L. Barron, Tamara Araújo, Márcio S.S. Pimenta, Paulo F.P. and Turco, Salvatore J. 2005. Leishmania braziliensis: a novel mechanism in the lipophosphoglycan regulation during metacyclogenesis. International Journal for Parasitology, Vol. 35, Issue. 3, p. 245.

Kohl, Linda and Bastin, Philippe 2005. A Survey of Cell Biology. Vol. 244, Issue. , p. 227.

Al-Mohammed, Hamdan I. Chance, Michael L. and Bates, Paul A. 2005. Production and Characterization of Stable Amphotericin-Resistant Amastigotes and Promastigotes of Leishmania mexicana . Antimicrobial Agents and Chemotherapy, Vol. 49, Issue. 8, p. 3274.

Saraiva, Elvira M. Pinto-da-Silva, Lucia H. Wanderley, João Luiz M. Bonomo, Adriana C. Barcinski, Marcello A. and Moreira, Maria Elisabete C. 2005. Flow cytometric assessment of Leishmania spp metacyclic differentiation: Validation by morphological features and specific markers. Experimental Parasitology, Vol. 110, Issue. 1, p. 39.

Kamhawi, Shaden 2006. Phlebotomine sand flies and Leishmania parasites: friends or foes?. Trends in Parasitology, Vol. 22, Issue. 9, p. 439.

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In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

Abstract

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This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Article contents

In vitro stimulation of metacyclogenesis in Leishmania braziliensis, L. donovani, L. major and L. mexicana

Abstract

Keywords

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