Hostname: page-component-745bb68f8f-cphqk Total loading time: 0 Render date: 2025-02-11T10:16:22.364Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of an Ixodes ricinus salivary gland fraction through its ability to stimulate CD4 T cells present in BALB/c mice lymph nodes draining the tick fixation site

Published online by Cambridge University Press:  01 July 1997

F. GANAPAMO ,
B. RUTTI  and
M. BROSSARD
F. GANAPAMO
Affiliation:
Present address: University of New Mexico, School of Medicine, Department of Microbiology and Immunology, 915 Camino de Salud NE, Albuquerque, NM 87131, USA. Institute of Zoology, Department of Immunology, University of Neuchâtel, Rue Emile Argand 11, 2007 Neuchâtel, Switzerland
B. RUTTI
Affiliation:
Institute of Zoology, Department of Immunology, University of Neuchâtel, Rue Emile Argand 11, 2007 Neuchâtel, Switzerland
M. BROSSARD
Affiliation:
Institute of Zoology, Department of Immunology, University of Neuchâtel, Rue Emile Argand 11, 2007 Neuchâtel, Switzerland
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

BALB/c mice infested with larvae or nymphs of Ixodes ricinus develop in their lymph nodes a T cell-specific immune response triggered by salivary gland soluble antigens (SGA). SGA are apparently conserved in the 3 biological stages of I. ricinus ticks and are species specific. SGA derived from partially fed females I. ricinus stimulate lymph node T cells from mice infested with I. ricinus larvae or nymphs. In contrast, lymph node cells from mice infested with Amblyomma hebraeum nymphs do not respond. A chromatographic fraction enriched with a 65 kDa protein (IrSG65) isolated from salivary glands of I. ricinus partially fed females induces in vitro a specific T cell proliferation of lymph node cells from mice infested with I. ricinus nymphs. The depletion of CD4+ T cells drastically reduces the ability of lymphocytes from infested mice to proliferate after IrSG65 stimulation.

Keywords

tickIxodes ricinussalivary glandTh cellsimmunogen
Type
Research Article
Information
Parasitology , Volume 115 , Issue 1 , July 1997 , pp. 91 - 96
Copyright
1997 Cambridge University Press