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A female-specific cDNA sequence of Schistosoma mansoni encoding an amidase that is expressed in the gastrodermis

Published online by Cambridge University Press:  01 February 1998

P. SCHÜßLER ,
K. KÖHRER ,
M. FINKEN-EIGEN ,
A. MICHEL ,
C. G. GREVELDING  and
W. KUNZ
P. SCHÜßLER
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
K. KÖHRER
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
M. FINKEN-EIGEN
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
A. MICHEL
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
C. G. GREVELDING
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
W. KUNZ
Affiliation:
Institute for Genetics and Center for Biological and Medical Research, Heinrich-Heine-University, Universitätsstrasse 1, D-40225 Düsseldorf, Germany
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Abstract

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A female-specifically expressed cDNA clone was obtained by screening of a subtractive cDNA library enriched for RNA from Schistosoma mansoni females. The deduced protein shows significant homology to a class of enzymes functioning as amidases. Northern blots revealed a transcript of 4·0 kb which is absent in larval stages, but is expressed in adult female worms. By in situ hybridization, the expression site of the gene was exclusively localized in the gastrodermis of female schistosomes. This is the first report of a female-specifically transcribed sequence of S. mansoni that is not expressed in the reproductive organs.

Keywords

Schistosoma mansoniamidasefemale-specific cDNA sequencegastrodermisgut epithelium
Type
Research Article
Information
Parasitology , Volume 116 , Issue 2 , February 1998 , pp. 131 - 137
Copyright
1998 Cambridge University Press

Footnotes

The sequence reported herein has been submitted to EMBL Data Library and assigned the accession number Y12802.