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The effect of praziquantel treatment on glutathione concentration in Schistosoma mansoni

Published online by Cambridge University Press:  01 March 1998

F. RIBEIRO ,
P. M. Z. COELHO ,
L. Q. VIEIRA ,
D. G. WATSON  and
J. R. KUSEL
F. RIBEIRO
Affiliation:
Department of Biochemistry and Molecular Biology, IBLS, Glasgow University, Glasgow G12 8QQ
P. M. Z. COELHO
Affiliation:
Department of Biochemistry and Molecular Biology, IBLS, Glasgow University, Glasgow G12 8QQ Present address: Departamento de Parasitologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
L. Q. VIEIRA
Affiliation:
Department of Biochemistry and Molecular Biology, IBLS, Glasgow University, Glasgow G12 8QQ Present address: Departamento de Bioquimica e Imunologia, Institutio de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
D. G. WATSON
Affiliation:
Department of Biochemistry and Molecular Biology, IBLS, Glasgow University, Glasgow G12 8QQ Present address: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, Scotland.
J. R. KUSEL
Affiliation:
Department of Biochemistry and Molecular Biology, IBLS, Glasgow University, Glasgow G12 8QQ
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Abstract

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A fluorescent dye monochlorobimane (MCB) that binds glutathione (GSH) was used as a tool for measuring the concentration of GSH in skin and mechanically-transformed schistosomula. The specificity of MCB binding to GSH was confirmed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The MCB binding to GSH is an energy-dependent process since no labelling could be seen at low temperature. When 24-h-old schistosomula were depleted of GSH by buthionine sulfoximine (a specific inhibitor of GSH synthesis) for 18 h, a significant decrease (P<0·001) in fluorescence was observed. PZQ treatment of the schistosomula after first labelling the parasites with MCB did not greatly affect MCB binding to GSH. However, when the 24-h-old schistosomula were first PZQ treated and afterwards labelled with MCB, the pattern of labelling was identical to that of those of the non-labelled parasites. When 24-h-old schistosomula were first PZQ treated, washed and labelled in the presence of 1 mM GSH, the level of fluorescence was recovered. These results suggest that PZQ depletes GSH from schistosomula, and may render them susceptible to the host's immune system.

Keywords

Schistosoma mansonipraziquantelglutathionemonochlorobimanebuthionine sulfoximine
Type
Research Article
Information
Parasitology , Volume 116 , Issue 3 , March 1998 , pp. 229 - 236
Copyright
1998 Cambridge University Press