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Comparison of the intergenic spacers and 3′ end regions of the large subunit (28S) ribosomal RNA gene from three species of Schistosoma

Published online by Cambridge University Press:  01 September 1998

R. A. KANE  and
D. ROLLINSON
R. A. KANE
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
D. ROLLINSON
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
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Abstract

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The sequences for the entire intergenic spacer (IGS) and 3′ end region of the large subunit (28S) ribosomal RNA gene are presented for Schistosoma intercalatum, S. haematobium and S. mansoni. The IGS was found to have at least 2 size formats for S. intercalatum and S. haematobium and the region for all 3 species contains numerous repeats and evidence of recombination. An A+T rich sequence found in S. intercalatum and S. haematobium is described and its possible function and origins are discussed. The amplification of this region by means of the polymerase chain reaction can be used to discriminate clearly between the 3 species involved in this study. The putative end of the 28S gene is identified.

Keywords

intergenic spacerrRNAA+T rich sequencerepeatsSchistosoma intercalatumSchistosoma haematobiumSchistosoma mansoni
Type
Research Article
Information
Parasitology , Volume 117 , Issue 3 , September 1998 , pp. 235 - 242
Copyright
1998 Cambridge University Press