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Cloning, heterologous expression and antigenicity of a schistosome cercarial protease

Published online by Cambridge University Press:  01 May 1997

H. P. PRICE ,
M. J. DOENHOFF  and
J. R. SAYERS
H. P. PRICE
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
M. J. DOENHOFF
Affiliation:
School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK
J. R. SAYERS
Affiliation:
University of Sheffield, Section of Molecular Medicine, Sheffield S10 2JF, UK
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Abstract

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A gene coding for the 30 kDa Schistosoma mansoni cercarial protease was amplified using the polymerase chain reaction (PCR) from genomic DNA templates. Cloning and sequencing of several independent PCR clones revealed the presence of an intron additional to the one described in the original cloning of the gene. The 3 exons were cloned into expression vectors so that they could be expressed as separate glutathione-S-transferase (GST) translational fusions. Recombinant bacteria carrying these expression plasmids expressed the fusion proteins at high levels. Western blotting of bacterial lysates with sera raised against the native S. mansoni cercarial protease showed that all 3 exons were recognized. Thus we have produced recombinant bacteria capable of providing large amounts of an S. mansoni antigen for immunological studies and evaluation as a candidate vaccine.

Keywords

Schistosoma mansonicercarial-elastaseexpressionrecombinant-fusion-proteins
Type
Research Article
Information
Parasitology , Volume 114 , Issue 5 , May 1997 , pp. 447 - 453
Copyright
1997 Cambridge University Press