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Micropatterned Biorecognition Surfaces on Nonbiofouling Polymer by Living Radical Photopolymerization for High Sensitivity Biosensing

Published online by Cambridge University Press:  01 February 2011

Madoka Takai ,
James Sibarani  and
Kazuhiko Ishihara
Madoka Takai
Affiliation:
takai@mpc.t.u-tokyo.ac.jp, The University of Tokyo, Materials Engineering, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-8656, Japan
James Sibarani
Affiliation:
james@mpc.t.u-tokyo.ac.jp, The University of Tokyo, Department of Materials Engineering, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-8656, Japan
Kazuhiko Ishihara
Affiliation:
ishihara@mpc.t.u-tokyo.ac.jp, The University of Tokyo, Department of Materials Engineering, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-8656, Japan
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Abstract

Preparation of nonbiofouling surface and micropatterned biorecognition layer over nonbiofouling for highly sensitive microarray biosensors, was conducted by constructing biocompatible poly(2-methacryloyloxyethyl phosphorylcholine(MPC)) brushes on polymer substrates based on living radical polymerization using macrophotoiniferters comprised of 2-ethylhexyl methacrylate (EHMA) and 4-vinylbenzyl N,N-diethyldithiocarbamate (VBDC). Protein adsorption on these modified surfaces significantly reduces compared to on polymeric substrate and the micropatterning consisted of poly(MPC) brush and biorecognition layer can be easily done by applying photomask with high fidelity.

Keywords

biomaterialpolymerizationsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1093: Symposium CC – Designer Biointerfaces , 2008 , 1093-CC01-04
Copyright
Copyright © Materials Research Society 2008

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References

1. Senaratne, W., Andruzzi, L., and Ober, C.K., Biomacromolecules, 6, 2427 (2005).Google Scholar
2. Zhang, S., Benmakroha, Y., Rolfe, P., Tanaka, S., and Ishihara, K., Biosensors and Bioelectron, 11, 1019–1029 (1996).Google Scholar
3. Park, J-W, Kurosawa, S., Aizawa, H., Goda, Y., Takai, M., and Ishihara, K., Analyst, 131, 155–162 (2006).Google Scholar
4. Nakayama, Y. and Matsuda, T., Macromolecules, 29, 8622–8630 (1996).Google Scholar
5. Nishizawa, K., Konno, T., Takai, M., and Ishihara, K., Biomacromolecules, 9, 403–407 (2008).Google Scholar
6. Goto, Y., Matsuno, R., Konno, T., Takai, M., and Ishihara, K., Biomacromolecules, 9, 828–833 (2008).Google Scholar
7. Feng, W., Brash, J. L., and Zhu, S., Biomaterials, 27 847–855 (2006).Google Scholar