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Label-Free, Electrical Biomarker Detection Based on Nanowire Biosensors Utilizing Antibody Mimics as Capture Probes

Published online by Cambridge University Press:  28 January 2011

Hsiao-Kang Chang ,
Fumiaki Ishikawa  and
Chongwu Zhou
Hsiao-Kang Chang
Affiliation:
Department of Electrical Engineering, University of Southern California Los Angeles, CA 90089, U.S.A.
Fumiaki Ishikawa
Affiliation:
Department of Electrical Engineering, University of Southern California Los Angeles, CA 90089, U.S.A.
Chongwu Zhou
Affiliation:
Department of Electrical Engineering, University of Southern California Los Angeles, CA 90089, U.S.A.
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Abstract

Antibody mimic proteins (AMPs) are poly-peptides that bind to their target analytes with high affinity and specificity, just like conventional antibodies, but are much smaller in size (2-5 nm, less than 10kDa). In this report, we describe the first application of AMP in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at sub-nanomolar concentration in the presence of 44 μM bovine serum albumin as a background. Furthermore, negative control experiment is carried out to confirm the role of AMPs in N protein detection.

Keywords

biomedicalsensornanostructure
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1302: Symposium W – Nanowires—Growth and Device Assembly for Novel Applications , 2012 , mrsf10-1302-w05-08
Copyright
Copyright © Materials Research Society 2011

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References

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