Species identification of bivalve larvae using random amplified polymorphic DNA (RAPD): differentiation between Cerastoderma edule and C. lamarcki

C. André; M. Lindegarth; P.R. Jonsson; P. Sundberg

doi:10.1017/S0025315498000691

Abstract

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The polymerase chain reaction (PCR) was used to produce species-specific DNA markers (RAPDs) from two sibling cockle species and five other co-occurring intertidal bivalves. Amplification reactions with one single primer readily distinguished larvae and adults of Cerastoderma edule from larvae and adults of C. lamarcki, and from adults of Mya arenaria, Macoma balthica, Scrobicularia plana, Venerupis pulastra and Mytilus edulis. Random amplified polymorphic DNA (RAPD) is suggested as a simple and quick method to determine species identity in taxa that are difficult to identify on the basis of morphological characters alone, such as marine bivalve larvae.

Crossref Citations

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Féral, Jean-Pierre 2002. How useful are the genetic markers in attempts to understand and manage marine biodiversity?. Journal of Experimental Marine Biology and Ecology, Vol. 268, Issue. 2, p. 121.

Rego, Ignacio Martínez, Andrés González-Tizón, Ana Vieites, Juan Leira, Francisco and Méndez, Josefina 2002. PCR Technique for Identification of Mussel Species. Journal of Agricultural and Food Chemistry, Vol. 50, Issue. 7, p. 1780.

Fernández, Alicia García, Teresa González, Isabel Asensio, Luis Rodríguez, Miguel Ángel Hernández, Pablo E. and Martín, Rosario 2002. Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Analysis of a 16S rRNA Gene Fragment for Authentication of Four Clam Species. Journal of Food Protection, Vol. 65, Issue. 4, p. 692.

Schneider, Daniel W. Stoeckel, James A. Rehmann, Chris R. Douglas Blodgett, K. Sparks, Richard E. and Padilla, Dianna K. 2003. A developmental bottleneck in dispersing larvae: implications for spatial population dynamics. Ecology Letters, Vol. 6, Issue. 4, p. 352.

Smith, P.J. 2005. Stock Identification Methods. p. 371.

Freire, Ruth Insua, Ana and Méndez, Josefina 2005. Cerastodermaglaucum5S ribosomal DNA: characterization of the repeat unit, divergence with respect toCerastoderma edule, and PCR–RFLPs for the identification of both cockles. Genome, Vol. 48, Issue. 3, p. 427.

Vadopalas, Brent Bouma, Josh V. Jackels, Chemine R. and Friedman, Carolyn S. 2006. Application of real-time PCR for simultaneous identification and quantification of larval abalone. Journal of Experimental Marine Biology and Ecology, Vol. 334, Issue. 2, p. 219.

SCHMIDT, ALFONSO J. TORO, JORGE E. and CHAPARRO, OSCAR R. 2006. REPRODUCTIVE PATTERNS AND THEIR INFLUENCE ON THE POPULATION GENETICS OF SYMPATRIC SPECIES OF THE GENUS CREPIDULA (GASTROPODA: CALYPTRAEIDAE). Journal of Shellfish Research, Vol. 25, Issue. 2, p. 371.

Wang, Shi Bao, Zhenmin Zhang, Lingling Li, Ning Zhan, Aibin Guo, Wenbo Wang, Xiaolong and Hu, Jingjie 2006. A new strategy for species identification of planktonic larvae: PCR–RFLP analysis of the internal transcribed spacer region of ribosomal DNA detected by agarose gel electrophoresis or DHPLC. Journal of Plankton Research, Vol. 28, Issue. 4, p. 375.

Santaclara, Francisco J. Espiñeira, Montserrat and Vieites, Juan M. 2007. Molecular Detection of Xenostrobus securis and Mytillus Galloprovincialis Larvae in Galician Coast (Spain). Marine Biotechnology, Vol. 9, Issue. 6, p. 722.

Lewis, Ceri and Karageorgopoulos, Perikles 2008. A new species of Marphysa (Eunicidae) from the western Cape of South Africa. Journal of the Marine Biological Association of the United Kingdom, Vol. 88, Issue. 2, p. 277.

Zhan, Aibin Hu, Jingjie Hu, Xiaoli Lu, Wei Wang, Mingling Peng, Wei Hui, Min and Bao, Zhenmin 2008. Fast identification of scallop adductor muscles using species-specific microsatellite markers. European Food Research and Technology, Vol. 227, Issue. 2, p. 353.

Sawada, Hideki Saito, Hajime Hosoi, Masatomi and Toyohara, Haruhiko 2008. Evaluation of PCR methods for fixed bivalve larvae. Journal of the Marine Biological Association of the United Kingdom, Vol. 88, Issue. 7, p. 1441.

Freire, R. Arias, A. Mendez, J. and Insua, A. 2010. Sequence variation of the internal transcribed spacer (ITS) region of ribosomal DNA in Cerastoderma species (Bivalvia: Cardiidae). Journal of Molluscan Studies, Vol. 76, Issue. 1, p. 77.

Freire, Ruth Arias, Alberto Méndez, Josefina and Insua, Ana 2011. Identification of European commercial cockles (Cerastoderma edule and C. glaucum) by species-specific PCR amplification of the ribosomal DNA ITS region. European Food Research and Technology, Vol. 232, Issue. 1, p. 83.

Heaney, S.A. Maloy, A.P. and Slater, J.W. 2011. Evaluation of fixatives and autofluorescence reduction treatments for marine bivalve larvae. Journal of the Marine Biological Association of the United Kingdom, Vol. 91, Issue. 7, p. 1567.

Malham, Shelagh K. Hutchinson, Thomas H. and Longshaw, Matt 2012. A review of the biology of European cockles (Cerastodermaspp.). Journal of the Marine Biological Association of the United Kingdom, Vol. 92, Issue. 7, p. 1563.

Morgan, E O’Riordan, RM Kelly, TC and Culloty, SC 2012. Influence of disseminated neoplasia, trematode infections and gametogenesis on surfacing and mortality in the cockle Cerastoderma edule. Diseases of Aquatic Organisms, Vol. 98, Issue. 1, p. 73.

Jensen, Pamela C. Purcell, Maureen K. Morado, J. Frank and Eckert, Ginny L. 2012. Development of a Real-Time Pcr Assay for Detection of Planktonic Red King Crab (Paralithodes camtschaticus(Tilesius 1815)) Larvae. Journal of Shellfish Research, Vol. 31, Issue. 4, p. 917.

Macali, Armando Conde, Anxo Smriglio, Carlo Mariottini, Paolo and Crocetta, Fabio 2013. The evolution of the molluscan biota of Sabaudia Lake: a matter of human history. Scientia Marina, Vol. 77, Issue. 4, p. 649.

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Species identification of bivalve larvae using random amplified polymorphic DNA (RAPD): differentiation between Cerastoderma edule and C. lamarcki

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