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Effectiveness of alternative organic solvents in field preservation of whole barnacles for PCR analyses

Published online by Cambridge University Press:  12 August 2004

P.L.M. Lee  and
C.M. Beynon
P.L.M. Lee
Affiliation:
School of Biological Sciences, University of Wales Swansea, Swansea, SA2 8PP, UK
C.M. Beynon
Affiliation:
Clinical School, University of Wales Swansea, Swansea, SA2 8PP, UK
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Abstract

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There are few reports of non-cryogenic preservation methods for marine invertebrates, so potable alcohol and acetone-based nail varnish remover (NVR) are for the first time evaluated against absolute ethanol as short-term preservatives of whole barnacles. Performance of ethanol and NVR-preserved material was comparable, but potable alcohol was significantly worse. These results are of practical importance for fieldwork in remote areas where laboratory chemicals are unattainable but potable alcohol or NVR are locally available. Of these, acetone-based NVR would be the solvent of preference.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 84 , Issue 4 , August 2004 , pp. 747 - 749
Copyright
2004 Marine Biological Association of the United Kingdom