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Adaptation of an immunodot assay for multiple prey identification of squid paralarvae in field trials

Published online by Cambridge University Press:  09 November 2005

H.J.T. Hoving ,
J.D. Venter ,
D.E. Worst  and
M.R. Lipinski
H.J.T. Hoving
Affiliation:
University of Groningen, Biological Centre, Department of Marine Biology, PO Box 14, 9750 AA Haren (Gn), The Netherlands
J.D. Venter
Affiliation:
Medical Research Council Private Bag X385, Pretoria 001, Republic of South Africa
D.E. Worst
Affiliation:
University of Groningen, Biological Centre, Department of Marine Biology, PO Box 14, 9750 AA Haren (Gn), The Netherlands
M.R. Lipinski
Affiliation:
Marine and Coastal Management Private Bag X2, Roggebaai 8012, Cape Town 8000, Republic of South Africa
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Abstract

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An optimized method, using polyclonal antibodies in an immunoassay, for prey detection in the diet of paralarvae of South African Loligo reynaudii is described. The study has increased the specificity of the antisera by determining the optimum antiserum dilutions and the detection limits of the antisera. Unfed laboratory-hatched paralarvae (negative control) were exposed to antisera and showed cross-reactions with polychaete antiserum.

Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 85 , Issue 6 , December 2005 , pp. 1499 - 1501
Copyright
© 2005 Marine Biological Association of the United Kingdom