An assemblage of shallow-water fish was sampled with a small beach seine over monthly 24-h periods between April 2000 and March 2001 at the sandy beach Duće Glava in the eastern Adriatic. Monthly sets of samples were divided into day and night catches to examine the stability of diel differences in assemblage structure over a 1-y period. A total of 61 species was caught, of which six were exclusively diurnal and 12 were nocturnal. According to abundance and biomass of individuals during day and night, the most abundant species were categorized into several groups. Ophidion rochei was exclusively nocturnal, while Nerophis ophidion and Echiichthys vipera were mostly nocturnal. Diplodus annularis and Mullus surmuletus were slightly nocturnal. Diplodus vulgaris, Pomatoschistus marmoratus and Atherina boyeri lacked a diel pattern. Sardina pilchardus, Lithognathus mormyrus, Atherina hepsetus, Sarpa salpa and Mullus surmuletus abundance peaked for a few months, probably related to timing of spawning and recruitment. At the assemblage level, the diel per cent similarity index indicates that there were major differences between the day and night assemblages in April, September, March, and August with respect to number of individuals and September, October, May and March with respect to biomass.