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Molecular approaches for studying ascaridoid nematodes with zoonotic potential, with an emphasis on Toxocara species

Published online by Cambridge University Press:  07 February 2017

X.Q. Zhu ,
R.B. Gasser ,
N.B. Chilton  and
D.E. Jacobs
X.Q. Zhu*
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway Werribee, Victoria 3030, Australia
R.B. Gasser
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway Werribee, Victoria 3030, Australia
N.B. Chilton
Affiliation:
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway Werribee, Victoria 3030, Australia
D.E. Jacobs
Affiliation:
Department of Pathology and Infectious Diseases, The Royal Veterinary College, University of London, North Mymms, Hatfield AL9 7TA, UK
*
* Corresponding author Fax: +613 97312366 E-mail: x.zhu@vet.unimelb.edu.au
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Abstract

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Species-specific identification of ascaridoid nematodes at any developmental stage is a prerequisite for detailed investigation of the life cycles, systematics and epidemiology of this important group, and is also crucial for the diagnosis of associated infections. The morphological identification of some species and/ or their larval stages can, however, present considerable difficulty. Recently, PCR-based methods, using genetic markers in the internal transcribed spacers (ITS) of ribosomal DNA, have been shown to provide reliable alternatives to more traditional methods for the specific identification of nematodes. This article provides an account of recent research on the development of PCR-based methods (utilizing ITS sequences) for the specific identification of ascaridoid nematodes of zoonotic potential, for the diagnosis of infections, and for the analysis of genetic variation within and among individual nematodes and their populations. Prospects for using these diagnostic and analytical tools to investigate epidemiological and population genetic questions relating to ascaridoid parasites are also discussed.

Type
Research Article
Information
Journal of Helminthology , Volume 75 , Issue 2 , June 2001 , pp. 101 - 108
Copyright
Copyright © Cambridge University Press 2001