The existence and magnitude of slip velocities between deionized water and a smooth glass surface is studied experimentally. Sub-micron fluorescent particles are suspended in water and imaged using total internal reflection velocimetry (TIRV). For water flowing over a hydrophilic surface, the measurements are in agreement with previous experiments and indicate that slip, if present, is minimal at low shear rates, but increases slightly as the shear rate increases. Surface hydrophobicity is observed to induce a small slip velocity, with the slip length reaching a maximum of 96 nm at a shear rate of 1800$\,{\rm s}^{-1}$. Issues associated with the experimental technique and the interpretation of results are also discussed.