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Trypsin-inhibitors in mastitic milk and colostrum: correlation between trypsin-inhibitor capacity, bovine serum albumin and somatic cell contents

Published online by Cambridge University Press:  01 June 2009

Tuula Honkenen-Buzalski  and
Markus Sandholm
Tuula Honkenen-Buzalski
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland
Markus Sandholm
Affiliation:
Department of Pharmacology and Toxicology and Department of Medicine, College of Veterinary Medicine, Helsinki 55, Finland and State Veterinary Medical Institute, Helsinki 55, Finland
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Summary

The trypsin-inhibitor capacity of bovine milk was seen to increase in mastitits. This capacity showed good correlation with the California Mastitis Test score, somatic cell count (Coulter Counter method) and bovine serum albumin (BSA) content (radial immunodiffusion). The elevated antitrypsin level proved to be a safer indicator of mastitis than BSA alone. Using gel-filtration chromatography, the major trypsin-inhibitor activity of mastitic milk and plasma was eluted with BSA, indicating a similar mol. wt (70000). This fraction showed electrophoretic polymorphism. A large molecular weight fraction cross-reacted immunologically with human α2-macroglobulin. The antitrypsin activity in mastitic milk was clearly different from the principal activity in colostrum, which had a mol. wt of about 13000.

Type
Original Articles
Information
Journal of Dairy Research , Volume 48 , Issue 2 , June 1981 , pp. 213 - 223
Copyright
Copyright © Proprietors of Journal of Dairy Research 1981

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