Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

CHRISTOPHER M. GOODING; PRABHAKARA V. CHOUDARY

doi:10.1017/S0022029996001896

Abstract

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Escherichia coli O157[ratio ]H7 in spiked samples of raw milk and ice-cream was enriched in tryptic soy broth for 4 h, captured by immunomagnetic separation, subjected to amplification by polymerase chain reaction of parts of the verotoxin genes (SLT-I and SLT-II), and detected by agarose gel electrophoresis. Using this method, as few as 1 cfu Esch. coli O157[ratio ]H7/g food could be detected in <10 h.

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Rapid and sensitive immunomagnetic separation–polymerase chain reaction method for the detection of Escherichia coli O157[ratio ]H7 in raw milk and ice-cream

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