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A new method for the preparation of an immunologically homogeneous β-casein

Published online by Cambridge University Press:  01 June 2009

J. Garnier ,
B. Ribadeau-Dumas  and
G. Mocquot
J. Garnier
Affiliation:
Station Centrale de Microbiologie et Recherches LaitièresI.N.R.A., Jouy-en-Josas (S. & 0.) France
B. Ribadeau-Dumas
Affiliation:
Station Centrale de Microbiologie et Recherches LaitièresI.N.R.A., Jouy-en-Josas (S. & 0.) France
G. Mocquot
Affiliation:
Station Centrale de Microbiologie et Recherches LaitièresI.N.R.A., Jouy-en-Josas (S. & 0.) France
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Summary

The preparation of an immunologically homogeneous β-casein is described, involving several separations by chromatography on diethylaminoethyl cellulose with urea-imidazole buffer at pH 7, followed by Tris buffer at pH 8·2. 30% of the β-casein present in the skim-milk is obtained by this method. β-casein gives a single band in urea-starch gel electrophoresis when it is obtained from the milk of cows homozygous for this character.

Type
Original Articles
Information
Journal of Dairy Research , Volume 31 , Issue 1 , February 1964 , pp. 131 - 136
Copyright
Copyright © Proprietors of Journal of Dairy Research 1964

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References

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