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Determination of bovine lactoferrin in lactoferrin-supplemented dairy products and raw milk by an automated latex assay

Published online by Cambridge University Press:  21 September 2006

Takashi Soejima ,
Koji Yamauchi ,
Tomoko Yamamoto ,
Yasuko Ohara ,
Eiji Nagao ,
Keiichi Kanbara ,
Munetoshi Fujisawa ,
Yuko Okuda  and
Shyunji Namba
Takashi Soejima
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Koji Yamauchi
Affiliation:
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Tomoko Yamamoto
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Yasuko Ohara
Affiliation:
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Eiji Nagao
Affiliation:
Analytical Research Center, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama, Kanagawa 228-8583
Keiichi Kanbara
Affiliation:
Bio Links Co. Ltd, 18-6, Takayama, Tuzuki-ku, Yokohama, Kanagawa 224-0065
Munetoshi Fujisawa
Affiliation:
Bio Links Co. Ltd, 18-6, Takayama, Tuzuki-ku, Yokohama, Kanagawa 224-0065
Yuko Okuda
Affiliation:
Department of Laboratory Medicine, Toho University Ohmori Hospital, 6-11-1, Ohmorinishi, Ohta-ku, Tokyo 143-8541, Japan
Shyunji Namba
Affiliation:
Department of Laboratory Medicine, Toho University Ohmori Hospital, 6-11-1, Ohmorinishi, Ohta-ku, Tokyo 143-8541, Japan
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Abstract

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Latex immune agglutination method with a multipurpose auto-analyser (the automated latex assay) was validated for determination of bovine lactoferrin (BLF) in various dairy products. Reproducibility-within-laboratory (intermediate precision) due to day for infant formula, UHT milk and yogurt supplemented with BLF at 50 mg/100 g for infant formula and UHT milk, and at 100 mg/100 g for yogurt were 1·62 to 3·10 mg/100 g. Reproducibility-within-laboratory due to analysis (morning, noon, and evening) for raw milk was 1·59 mg/100 g. Trueness, accuracy in determining known amounts added for BLF-supplemented dairy products was −4·7 to −2·0 mg/100 g. BLF concentration in raw milks was 20·3 to 21·8 mg/100 g. Although interference by the matrixes took place in infant formula and raw milk, BLF assays were accurately carried out by 2000-fold dilution or standard-addition method. Automated latex assay for BLF is simple, rapid, precise and accurate enough for a routine method in various dairy products containing BLF.

Keywords

Automated latex assaybovine lactoferrindairy productsdeterminationICHlatex agglutinationvalidation
Type
Research Article
Information
Journal of Dairy Research , Volume 74 , Issue 1 , February 2007 , pp. 100 - 105
Copyright
Proprietors of Journal of Dairy Research 2006