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Phylogenetic analysis of yeast in the rumen contents of cattle based on the 26S rDNA sequence

Published online by Cambridge University Press:  24 March 2005

E. C. SHIN ,
Y. K. KIM ,
W. J. LIM ,
S. Y. HONG ,
C. L. AN ,
E. J. KIM ,
K. M. CHO ,
B. R. CHOI ,
J. M. AN  and
J. M. KANG
...Show all authors
E. C. SHIN
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
Y. K. KIM
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
W. J. LIM
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
S. Y. HONG
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
C. L. AN
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
E. J. KIM
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
K. M. CHO
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
B. R. CHOI
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
J. M. AN
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
J. M. KANG
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
Y. J. JEONG
Affiliation:
Department of Agricultural Chemistry, Sunchon National University, Sunchon 540-742, Republic of Korea
E. J. KWON
Affiliation:
Department of Agricultural Chemistry, Sunchon National University, Sunchon 540-742, Republic of Korea
H. KIM
Affiliation:
Department of Agricultural Chemistry, Sunchon National University, Sunchon 540-742, Republic of Korea
H. D. YUN
Affiliation:
Division of Applied Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea Research Institute of Life Science, Gyeongsang National University, Chinju 660-701, Republic of Korea
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Abstract

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The composition of yeast communities in the rumen of cattle was investigated using comparative DNA sequence analysis of yeast 26S rDNA genes. 26S rDNA libraries were constructed from rumen fluid (FF), rumen solid (FS) and rumen epithelium (FE). A total of 97 clones, containing a partial 26S rDNA sequence of 0·6 kb length, were sequenced and subjected to an on-line similarity search.

The 41 FF clones could be divided into five classes. The largest class was affiliated with Pezizomycotina class (85·4% of clones), and the remaining classes were related with the Urediniomycotina (2·4%), Hymenomycetes (4·9%), Ustilaginomycetes (4·9%) and Saccharomycotina (2·4%) classes. The 26 FE clones could be divided into three classes and the Saccharomycetes class (92·4% of clones) was the largest group. The remaining classes were related with either Pezizomycotina (3·8%) or Ustilaginomycetes (3·8%). The 30 FS clones were all affiliated with Saccharomycotina. Saccharomycotina were predominant in rumen epithelium and rumen solid while Pezizomycotina were predominant in rumen fluid. Yeast belonging to the Saccharomycotina class was predominant in the rumen as a whole (57%). One clone (FF34) had less than 90% similarity to any sequence in the database and was thus apparently unrelated to any previously described yeast.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 142 , Issue 5 , October 2004 , pp. 603 - 611
Copyright
© 2004 Cambridge University Press