Hostname: page-component-7b9c58cd5d-f9bf7 Total loading time: 0 Render date: 2025-03-16T06:38:19.744Z Has data issue: false hasContentIssue false

Detection and Prevalence of Adenoviral Conjunctivitis among Hospital Employees Using Real-Time Polymerase Chain Reaction as an Infection Prevention Tool

Published online by Cambridge University Press:  10 May 2016

Irene C. Kuo*
Affiliation:
Wilmer Eye Institute, Department of Ophthalmology, Johns Hopkins University School of Medicine, Baltimore, Maryland
Colleen Espinosa
Affiliation:
Division of Occupational and Environmental Medicine, Johns Hopkins Hospital, Baltimore, Maryland
Michael Forman
Affiliation:
Division of Medical Microbiology, Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland
Miriana Pehar
Affiliation:
Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, Maryland
Lisa L. Maragakis
Affiliation:
Department of Hospital Epidemiology and Infection Control, Johns Hopkins Hospital, Baltimore, Maryland Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland
Alexandra Valsamakis
Affiliation:
Division of Medical Microbiology, Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland
*
Wilmer Eye Institute, 4924 Campbell Boulevard, #100, Baltimore, MD 21236 (ickuo@jhmi.edu).
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Hospital employees with suspected adenoviral conjunctivitis underwent evaluation and testing with real-time polymerase chain reaction. Viral conjunctivitis was suspected in 307 (59%) of 518 employees with eye complaints; adenovirus was detected in 4% (22 of 518). Four employees had genotypes consistent with epidemic keratoconjunctivitis. This algorithm minimizes productivity loss compared with clinical diagnosis.

Infect Control Hosp Epidemiol 2014;35(6):728–731

Type
Original Articles
Copyright
© 2014 by The Society for Healthcare Epidemiology of America. All rights reserved.

References

1. Montessori, V, Scharf, S, Holland, S, Werker, DH, Roberts, FJ, Bryce, E. Epidemic keratoconjunctivitis outbreak at a tertiary referral eye care clinic. Am J Infect Control 1998;26(4):399405.Google Scholar
2. Cheung, D, Bremner, J, Chan, JTK. Epidemic keratoconjunctivitis—do outbreaks have to be epidemic? Eye 2003;17:356363.Google Scholar
3. Klapper, PE, Cleator, GM. Adenovirus cross-infection: a continuing problem. J Hosp Infect 1995;30(suppl):262267.Google Scholar
4. Hamada, N, Gotoh, K, Hara, K, et al. Nosocomial outbreak of epidemic keratoconjunctivitis accompanying environmental contamination with adenoviruses. J Hosp Infect 2008;68:262268.Google Scholar
5. Piednoir, E, Bureau-Chalot, F, Merle, C, Gotzmanis, A, Wulbout, J, Bajolet, O. Direct costs associated with a nosocomial outbreak of adenoviral conjunctivitis infection in a long-term care instititution. Am J Infect Control 2002;30:407410.Google Scholar
6. Dart, JKG, El-Amir, AN, Maddison, T, et al. Identification and control of nosocomial adenovirus keratoconjunctivitis in an ophthalmic department. Br J Ophthalmol 2009;93:1820.Google Scholar
7. Warren, D, Nelson, KE, Farrar, JA, et al. A large outbreak of epidemic keratoconjunctivitis. J Infec Dis 1989;160(6):938943.Google Scholar
8. Gottsch, JD, Froggatt, JW, Smith, DM, et al. Prevention and control of epidemic keratoconjunctivitis in a teaching eye institute. Ophthalmic Epidemiol 1999;6:2939.Google Scholar
9. Aoki, K, Kato, M, Ohtsuka, H, Ishii, K, Nakazono, N, Sawada, H. Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus type 4 and 19 infections. Br J Ophthalmol 1982;66:776780.Google Scholar
10. Aoki, K, Kawana, R, Matsumoto, I, Wadell, G, de Jong, JC. Viral conjunctivitis with special reference to adenovirus type 37 and enterovirus 70 infection. Jpn J Ophthalmol 1986;30:158164.Google Scholar
11. Aoki, K, Tagawa, Y. A twenty-one year surveillance of adenoviral conjunctivitis in Sapporo, Japan. Int Ophthalmol Clin 2002:42:4954.Google Scholar
12. de Jong, JC, Wigand, R, Wadell, G, et al. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J Med Virol 1981;7:105118.Google Scholar
13. Desmyter, J, De Jong, JC, Slaterus, KW, Verlaeckt, H. Keratoconjunctivitis caused by adenovirus type 19. Br Med J 1974; 4:406.Google Scholar
14. Jawetz, E, Kimura, S, Nicholas, AN, Thygeson, P, Hanna, P. New type of APC virus from epidemic keratoconjunctivitis. Science 1955;122:11901191.Google Scholar
15. Wold, WSM, Horwitz, MS. Adenoviridae. In: Knipe, DM, Howley, PM, Griffin, DE, Lamb, RA, Martin, MA, Roizman, B, Straus, SE, eds. Fields virology. 5th ed. Vol. II. Philadelphia: Lippincott, Williams & Wilkins, 2007:23952436.Google Scholar
16. Heim, A, Ebnet, C, Harste, G, Pring-Akerblom, P. Rapid and quantitative detection of human adenovirus DNA by real-time PCR. J Med Virol 2003;70:228239.Google Scholar
17. Lu, X, Erdman, DD. Molecular typing of human adenoviruses by PCR and sequencing of a partial region of the hexon gene. Arch Virol 2006;151:15871602.Google Scholar