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Identification of a second locus encoding β-amylase on chromosome 2 of barley

Published online by Cambridge University Press:  14 April 2009

M. Kreis ,
M. S. Williamson ,
P. R. Shewry ,
P. Sharp  and
M. Gale
M. Kreis*
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
M. S. Williamson
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
P. R. Shewry
Affiliation:
AFRC Institute of Arable Crop Research, Biochemistry Department, Rothamsied Experimental Station, Harpenden, Herts, AL5 2JQ, U.K.
P. Sharp
Affiliation:
AFRC Institute of Plant Science Research, Plant Breeding Institute. Mans Lane, Trumpington, Cambridge CB2 2LQ., U.K.
M. Gale
Affiliation:
AFRC Institute of Plant Science Research, Plant Breeding Institute. Mans Lane, Trumpington, Cambridge CB2 2LQ., U.K.
*
* Corresponding authors.
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Summary

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A barley endosperm cDNA clone was used to study the polymorphism and chromosomal location of β-amylase genes in barley. Analysis of DNA from seven cultivars digested with three restriction endonucleases showed two types of pattern, one present in Sultan and the other in the remaining six cultivars. A copy-number reconstruction indicated the presence of about three gene copies per haploid genome. Analysis of the six available whole chromosome addition lines and selected telocentric chromosome additions of barley into wheat showed the location of genes on the short arm of chromosome 2 (probably one copy) and the long arm of chromosome 4 (probably two copies).

Type
Research Article
Information
Genetics Research , Volume 51 , Issue 1 , February 1988 , pp. 13 - 16
Copyright
Copyright © Cambridge University Press 1988

References

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