Hostname: page-component-6bf8c574d5-vmclg Total loading time: 0 Render date: 2025-02-14T15:39:24.569Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of morphogenetic processes in Paramecium: I. A mutation affecting trichocyst formation and nuclear division

Published online by Cambridge University Press:  14 April 2009

Françoise Ruiz ,
André Adoutte ,
Michèle Rossignol  and
Janine Beisson
Françoise Ruiz
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
André Adoutte
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Michèle Rossignol
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Janine Beisson
Affiliation:
Centre de Génétique Moléculaire, CNRS, 91190 Gif-sur-Yvette, France
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutation tam38 of Paramecium tetraurelia is a nuclear recessive mutation with a pleiotropic effect on both trichocyst morphogenesis and nuclear processes. The analysis of the defective nuclear processes (micronuclear and macronuclear divisions, nuclear reorganization at autogamy) shows that these defects result from an abnormal localization of the nuclei. Phenocopies of tam38 abnormalities can be obtained by vinblastine treatment of wild-type cells at late stages of division. Taking into account the similarity between tam38 and a series of other mutations which also prevent trichocyst attachment to the cell surface and disturb nuclear divisions, the following interpretation is proposed: the absence of attached trichocyst induces structural changes in the plasma membrane or in the cortical region which disturb the normal cortical control of the localization of nuclei.

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976 , pp. 109 - 122
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Adoutte, A. & Beisson, J. (1970). Cytoplasmic inheritance of erythromycin resistant mutations in Paramecium. Molecular and General Genetics 108, 70–77.CrossRefGoogle ScholarPubMed
Beisson, J. (1972). Déterminants nucléaires et cytoplasmiques dans la biogenèse des structures chez les protozoaires. Annales de Biologie 11, 401–411.Google Scholar
Beisson, J., Lefort-Tran, M., Pouphille, M., Rossignol, M. & Satir, B. (1976). Genetic analysis of membrane differentiation in Paramecium. Journal of Cell Biology (in the Press).CrossRefGoogle ScholarPubMed
Beisson, J. & Rossignol, M. (1975). Movements and positioning of organelles in Paramecium aurelia. In Nucleocytoplasmic Relationships during Cell Morphogenesis in Some Unicellular Organisms (ed. Puiseux-Dao, S.), pp. 291–294. Elsevier.Google Scholar
Bennet, D., Boyse, E. A. & Old, L. J. (1972). Cell surface immunogenetics in the study of morphogenesis. In Cell Interactions (ed. Silvestri, L.), pp. 247–263. North-Holland.Google Scholar
Berlin, R. D., Oliver, J. M., Ukena, T. E. & Yin, H. H. (1974). Control of cell surface topography. Nature 247, 45–46.Google Scholar
Berger, J. D. (1973). Selective inhibition of DNA synthesis in macronuclear fragments in Paramecium aurelia exconjugants and its reversal during macronuclear regeneration. Chromosoma 44, 33–48.CrossRefGoogle ScholarPubMed
Burger, M. (1973). Surface changes in transformed cells detected by lectins. Federation Proceedings 32, 91–101.Google ScholarPubMed
Cheng, K. & Katsoyannis, P. G. (1975). The inhibition of sugar transport and oxidation in fat cell ghosts by colchicine. Biochemical and Biophysical Research Communications 64, 1069–1075.CrossRefGoogle ScholarPubMed
Corliss, J. O. (1953). Silver impregnation of ciliated protozoa by the Chatton-Lwoff technique. Stain Technology 28, 97–100.Google Scholar
De Terra, N. (1971). Evidence for cortical control of macronuclear behavior in Stentor. Journal of Cellular Physiology 78, 377–386.Google Scholar
De Terra, N. (1973). Further evidence for cortical control over replication of the macronucleus and basal bodies in Stentor. Developmental Biology 32, 129–139.Google Scholar
De Terra, N. (1974). Cortical control of cell division. Science 184, 530–537.Google Scholar
Dipell, R. V. (1955). A temporary stain for Paramecium and other protozoa. Stain Technology 30, 69–71.CrossRefGoogle Scholar
Kaneda, M. & Hanson, E. D. (1974). Growth patterns and morphogenetic events in the cell cycle of Paramecium aurelia. In Paramecium, a Current Survey (ed. Van Wagtendonk, W. J.), pp. 219–261. Elsevier.Google Scholar
Mazia, D. (1974). The chromosome cycle in the cell cycle. In Cell Cycle Controls (ed. Padilla, G. M. et al. ), pp. 265–272. Academic Press.Google Scholar
Nanney, D. L. (1953). Nucleo-cytoplasmic interaction during conjugation in Tetrahymena. Biological Bulletin 105, 133–148.CrossRefGoogle Scholar
Olmsted, J. B. & Borisy, G. G. (1973). Microtubules. Annual Review of Biochemistry 42, 507–540.CrossRefGoogle ScholarPubMed
Pardee, A. B. (1971). The surface membrane as a regulator of animal cell division. In vitro 7, (2), 95–104.CrossRefGoogle Scholar
Pollack, S. (1974). Mutations affecting the trichocysts in Paramecium aurelia. I. Morphology and description of the mutants. Journal of Protozoology 21, 352–362.Google Scholar
Sonneborn, T. M. (1954 a). Gene controlled aberrant nuclear behavior in Paramecium aurelia. Microbial Genetics Bulletin 11, 24–25.Google Scholar
Sonneborn, T. M. (1954 b). Patterns of nucleocytoplasmic integration in Paramecium. Caryologia 6, 307–325.Google Scholar
Sonneborn, T. M. (1970). Methods in Paramecium research. Methods in Cell Physiology 4, 241–339.Google Scholar
Sonneborn, T. M. (1974 a). Ciliate morphogenesis and its bearing on general cellular morphogenesis. Actualités Protozoologiques (ed. de Puytorac, P. and Grain, J.) 1, 327–355.Google Scholar
Sonneborn, T. M. (1974 b). Paramecium aurelia. In Handbook of Genetics (ed. King, R.), 2, 469–594. Plenum Press.CrossRefGoogle Scholar
Sonneborn, T. M. (1975). The Paramecium aurelia complex of 14 sibling species. Transactions of the American Microscopical Society 94, 155–178.Google Scholar
Tamura, S., Tsuruhara, T. & Watanabe, Y. (1969). Action of nuclear microtubules in macronuclear division of Tetrahymena pyriformis. Experimental Cell Research 55, 351–358.Google Scholar
Whittle, J. R. & Chen-Shan, L. (1972). Cortical morphogenesis in Paramecium aurelia: mutants affecting cell shape. Genetical Research 19, 271–279.Google Scholar